Localization of human RNase Z isoforms: dual nuclear/mitochondrial targeting of the ELAC2 gene product by alternative translation initiation.
Localization of human RNase Z isoforms: dual nuclear/mitochondrial targeting of the ELAC2 gene product by alternative translation initiation.
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DOI：
10.1371/journal.pone.0019152
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发表时间：
2011-04-29
期刊：
影响因子：
3.7
通讯作者：
Rossmanith W
中科院分区：
文献类型：
--
作者：
Rossmanith W
RNase Z 是一种核酸内切酶，负责去除 tRNA 前体的 3' 延伸，这是 tRNA 生物发生中的一个重要步骤。人类细胞含有由 ELAC2 编码的长形式（RNase ZL）和短形式（RNase ZS；ELAC1）。我们通过与绿色荧光蛋白融合的蛋白质的表达来研究它们的亚细胞定位。 RNase ZS 存在于细胞质中，而 RNase ZL 位于细胞核和线粒体中。我们表明替代翻译起始是 RNase ZL 双重靶向的原因。由于 ELAC2 第一个 AUG 的不利环境，翻译显然也从第二个 AUG 开始，由此线粒体靶向序列丢失，蛋白质转而被路由至细胞核。我们的数据表明 RNase ZL 是参与核和线粒体 tRNA 3' 末端成熟的酶。
RNase Z is an endonuclease responsible for the removal of 3′ extensions from tRNA precursors, an essential step in tRNA biogenesis. Human cells contain a long form (RNase ZL) encoded by ELAC2, and a short form (RNase ZS; ELAC1). We studied their subcellular localization by expression of proteins fused to green fluorescent protein. RNase ZS was found in the cytosol, whereas RNase ZL localized to the nucleus and mitochondria. We show that alternative translation initiation is responsible for the dual targeting of RNase ZL. Due to the unfavorable context of the first AUG of ELAC2, translation apparently also starts from the second AUG, whereby the mitochondrial targeting sequence is lost and the protein is instead routed to the nucleus. Our data suggest that RNase ZL is the enzyme involved in both, nuclear and mitochondrial tRNA 3′ end maturation.
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