Preparatory studies of composite mesenchymal stem cell islets for application in intraportal islet transplantation.
Preparatory studies of composite mesenchymal stem cell islets for application in intraportal islet transplantation.
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DOI：
10.3109/03009734.2010.524320
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发表时间：
2011-03
影响因子：
3.4
通讯作者：
Magnusson PU
中科院分区：
文献类型：
--
作者：
Duprez IR;Johansson U;Nilsson B;Korsgren O;Magnusson PU
植入率低和不良免疫反应是影响胰岛移植成功率的主要因素。在这项研究中，我们研究了人类间充质干细胞（MSC）的能力，坚持人类胰岛和免疫调节和血液相互作用过程中的影响，在体外。通过悬浮共培养形成复合MSC-胰岛，共聚焦显微镜观察表型。通过体外葡萄糖刺激下胰岛素的动态释放来评价胰岛功能。利用混合淋巴细胞-胰岛反应（MLIR）和管道血循环模型作为体外工具，分析MSC对胰岛引发的先天性和适应性免疫反应的影响。MSC迅速粘附于胰岛并扩散覆盖胰岛表面。胰岛素表达和分泌用MSC涂层维持。与对照胰岛相比，MSC包被的胰岛在体外与血液的反应未受影响。骨髓间充质干细胞还能抑制MLIR时胰岛细胞诱导的淋巴细胞增殖。我们的结论是，它是可能的，以创建复合MSC-胰岛，使通过利用MSC的粘附能力的MSC的交付。这可能在优化胰岛移植中具有有益的免疫抑制作用。
Low engraftment and adverse immune reactions hamper the success rate of clinical islet transplantation. In this study, we investigated the capacity of human mesenchymal stem cells (MSCs) to adhere to human islets of Langerhans and their effects in immune modulation and during blood interactions in vitro. Composite MSC–islets were formed by suspension co-culture, and the phenotype was evaluated by confocal microscopy. Islet function was assessed by dynamic insulin release in response to glucose in vitro. Mixed lymphocyte–islet reactions (MLIR) and the tubing blood loop model were utilized as in vitro tools to analyse the effect of MSCs on the innate and adaptive immune reactions triggered by the islets. MSCs rapidly adhered to islets and spread out to cover the islet surface. Insulin expression and secretion were sustained with the MSC coating. MSC-coated islets showed unaffected reactions with blood in vitro in comparison to control islets. Furthermore, MSCs suppressed lymphocyte proliferation induced by islet cells in MLIR. We conclude that it is possible to create composite MSC–islets to enable delivery of the MSCs by utilizing the adhesive capacity of the MSCs. This could have beneficial immunosuppressive effects in optimizing pancreatic islet transplantation.
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DOI：
10.3109/15419069309095685
发表时间：
1993-01-01
期刊：
CELL ADHESION AND COMMUNICATION
影响因子：
--
作者：
CHICHESTER, CO;FERNANDEZ, M;MINGUELL, JJ
通讯作者：
MINGUELL, JJ
影响因子：
6.2
作者：
Eich, Torsten;Eriksson, Olof;Lundgren, Torbjorn
通讯作者：
Lundgren, Torbjorn
DOI：
10.4049/jimmunol.0900803
发表时间：
2009-07-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Fiorina P;Jurewicz M;Augello A;Vergani A;Dada S;La Rosa S;Selig M;Godwin J;Law K;Placidi C;Smith RN;Capella C;Rodig S;Adra CN;Atkinson M;Sayegh MH;Abdi R
通讯作者：
Abdi R
影响因子：
3.6
作者：
Li, Haihong;Fu, Xiaobing;Sun, Tongzhu
通讯作者：
Sun, Tongzhu
影响因子：
2.6
作者：
Bartholomew, A;Sturgeon, C;Hoffman, R
通讯作者：
Hoffman, R