Quartz Crystal Microbalance with Dissipation Monitoring of Dynamic Viscoelastic Changes of Tobacco BY-2 Cells under Different Osmotic Conditions.
Quartz Crystal Microbalance with Dissipation Monitoring of Dynamic Viscoelastic Changes of Tobacco BY-2 Cells under Different Osmotic Conditions.
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耗散石英晶体微天平监测烟草BY-2细胞在不同渗透条件下的动态粘弹性变化
DOI：
10.3390/bios11050136
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发表时间：
2021-04-27
期刊：
影响因子：
--
通讯作者：
Duan H
中科院分区：
文献类型：
--
作者：
Chen Z;Zhou T;Hu J;Duan H
植物细胞力学包括膨压和细胞壁力学性质，不仅决定着植物细胞的生长，而且反映了植物细胞在生物和非生物胁迫下的功能和结构变化。然而，目前还没有合适的技术允许非侵入性地和连续地监测活植物细胞的复杂机械特性。本工作采用具有耗散效应的石英晶体微天平（QCM-D）监测技术（3-9），动态监测了不同浓度甘露醇（CM）作用下，烟草BY-2细胞与带正电荷的N，N-二甲基-N-丙烯基-2-丙烯-1-氯化铵均聚物（PDADMAC）/SiO_2 QCM晶体的粘附以及渗透胁迫的后续效应。用细胞粘弹性指数（CVIn）（CVIn = ΔD_（max）/ΔF）表征BY-2细胞在不同渗透压条件下的粘弹性。我们的研究结果表明，较低的泛音QCM可以检测细胞壁和细胞骨架结构允许检测的plasmodulation现象，而较高的泛音只能检测细胞壁的机械性能。光学显微镜下观察细胞形态学变化，并与QCM结果进行了进一步的讨论。通过对细胞粘弹性的非破坏性动态监测，可以跟踪植物细胞在外界刺激（或应力）作用下所产生的力或细胞结构的动态变化，为植物细胞的表征和研究提供了新的途径。QCM-D可以绘制活细胞中不同细胞结构的粘弹特性，可以作为一种新的工具来测试植物细胞的力学性能。
The plant cell mechanics, including turgor pressure and wall mechanical properties, not only determine the growth of plant cells, but also reflect the functional and structural changes of plant cells under biotic and abiotic stresses. However, there are currently no appropriate techniques allowing to monitor the complex mechanical properties of living plant cells non-invasively and continuously. In this work, quartz crystal microbalance with dissipation (QCM-D) monitoring technique with overtones (3–9) was used for the dynamic monitoring of adhesions of living tobacco BY-2 cells onto positively charged N,N-dimethyl-N-propenyl-2-propen-1-aminiumchloride homopolymer (PDADMAC)/SiO2 QCM crystals under different concentrations of mannitol (CM) and the subsequent effects of osmotic stresses. The cell viscoelastic index (CVIn) (CVIn = ΔD⋅n/ΔF) was used to characterize the viscoelastic properties of BY-2 cells under different osmotic conditions. Our results indicated that lower overtones of QCM could detect both the cell wall and cytoskeleton structures allowing the detection of plasmolysis phenomena; whereas higher overtones could only detect the cell wall’s mechanical properties. The QCM results were further discussed with the morphological changes of the BY-2 cells by an optical microscopy. The dynamic changes of cell’s generated forces or cellular structures of plant cells caused by external stimuli (or stresses) can be traced by non-destructive and dynamic monitoring of cells’ viscoelasticity, which provides a new way for the characterization and study of plant cells. QCM-D could map viscoelastic properties of different cellular structures in living cells and could be used as a new tool to test the mechanical properties of plant cells.
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DOI：
10.3390/molecules25173950
发表时间：
2020-08-29
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Migoń D;Wasilewski T;Suchy D
通讯作者：
Suchy D
影响因子：
4.2
作者：
Coussement, Jonas R.;De Swaef, Tom;Steppe, Kathy
通讯作者：
Steppe, Kathy
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
5.6
作者：
Guerriero G;Hausman JF;Cai G
通讯作者：
Cai G
DOI：
10.1085/jgp.201411295
发表时间：
2015-05
期刊：
The Journal of general physiology
影响因子：
--
作者：
Haswell ES;Verslues PE
通讯作者：
Verslues PE