Nitric Oxide and Hydrogen Peroxide Mediate Wounding-Induced Freezing Tolerance through Modifications in Photosystem and Antioxidant System in Wheat.
Nitric Oxide and Hydrogen Peroxide Mediate Wounding-Induced Freezing Tolerance through Modifications in Photosystem and Antioxidant System in Wheat.
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一氧化氮和过氧化氢通过改变小麦的光系统和抗氧化系统介导受伤引起的冷冻耐受性
DOI：
10.3389/fpls.2017.01284
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发表时间：
2017
影响因子：
5.6
通讯作者：
Jiang D
中科院分区：
文献类型：
--
作者：
Si T;Wang X;Wu L;Zhao C;Zhang L;Huang M;Cai J;Zhou Q;Dai T;Zhu JK;Jiang D
机械伤害是由食草动物或人工和自然操作引起的常见应激，而其在适应广泛的非生物应激反应中的作用尚不清楚。本研究表明，局部机械损伤增强了小麦叶片的抗冻能力，同时信号分子过氧化氢（H2O2）和一氧化氮（NO）也在系统积累。药理学研究表明，用活性氧清除剂和NADPH氧化酶（呼吸爆发氧化酶同源物，RBOH）抑制剂预处理后，损伤诱导的NO合成基本上被阻止。相反，损伤诱导的H2O2积累对NO合成抑制剂和清除剂不敏感，说明H2O2在损伤信号转导途径中作用于NO的上游。细胞化学和维管组织定位证实rboh依赖性H2O2在损伤反应中起远距离信号作用。转录组分析显示，279个基因在伤害和冷冻处理中上调，而在单独冷冻处理中没有上调。重要的是，冷冻和损伤诱导的基因在“光合作用”和“信号”类别中显著富集。这些结果有力地支持了初级机械损伤诱导小麦抗冻能力的机制，即通过系统积累NO和H2O2，并进一步改变光系统和抗氧化系统。
Mechanical wounding is a common stress caused by herbivores or manual and natural manipulations, whereas its roles in acclimation response to a wide spectrum of abiotic stresses remain unclear. The present work showed that local mechanical wounding enhanced freezing tolerance in untreated systemic leaves of wheat plants (Triticum aestivum L.), and meanwhile the signal molecules hydrogen peroxide (H2O2) and nitric oxide (NO) were accumulated systemically. Pharmacological study showed that wounding-induced NO synthesis was substantially arrested by pretreatment with scavengers of reactive oxygen species and an inhibitor of NADPH oxidase (respiratory burst oxidase homolog, RBOH). On the contrary, wounding-induced H2O2 accumulation was not sensitive to NO synthetic inhibitors or scavenger, indicating that H2O2 acts upstream of NO in wounding signal transduction pathways. Cytochemical and vascular tissues localizations approved that RBOH-dependent H2O2 acts as long-distance signal in wounding response. Transcriptome analysis revealed that 279 genes were up-regulated in plants treated with wounding and freezing, but not in plants treated with freezing alone. Importantly, freezing- and wounding-induced genes were significantly enriched in the categories of “photosynthesis” and “signaling.” These results strongly supported that primary mechanical wounding can induce freezing tolerance in wheat through the systemic accumulation of NO and H2O2, and further modifications in photosystem and antioxidant system.
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影响因子：
7.3
作者：
Aldea, M;Hamilton, JG;DeLucia, EH
通讯作者：
DeLucia, EH
影响因子：
7.2
作者：
Bright, J;Desikan, R;Neill, SJ
通讯作者：
Neill, SJ
影响因子：
7.2
作者：
Fryer, MJ;Ball, L;Baker, NR
通讯作者：
Baker, NR
影响因子：
11.6
作者：
Bestwick, CS;Brown, IR;Mansfield, JW
通讯作者：
Mansfield, JW
影响因子：
6.9
作者：
Chaki M;Valderrama R;Fernández-Ocaña AM;Carreras A;Gómez-Rodríguez MV;Pedrajas JR;Begara-Morales JC;Sánchez-Calvo B;Luque F;Leterrier M;Corpas FJ;Barroso JB
通讯作者：
Barroso JB