Two RmlC homologs catalyze dTDP-4-keto-6-deoxy-D-glucose epimerization in Pseudomonas putida KT2440.
Two RmlC homologs catalyze dTDP-4-keto-6-deoxy-D-glucose epimerization in Pseudomonas putida KT2440.
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DOI：
10.1038/s41598-021-91421-x
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发表时间：
2021-06-07
影响因子：
4.6
通讯作者：
Lassak J
中科院分区：
文献类型：
--
作者：
Koller F;Lassak J
鼠李糖是一种重要的单糖，既是营养来源，也是原核糖蛋白和糖脂的组成部分。这些复合分子的产生需要提供活化的前体，例如以核苷酸糖的形式，如dTDP-β-l-鼠李糖（dTDP-l- rha）。dTDP-l-Rha是在一个保守的4步反应中合成的，通常由酶RmlABCD催化。完整的途径对于假单胞菌的适应性尤其重要，因为dTDP-l-Rha对于这些细菌中脯氨酸特异性翻译延伸因子EF-P的激活至关重要。在本研究范围内，我们研究了恶臭假单胞菌KT2440的dtdp -l- rhai生物合成途径，重点研究了最后两个步骤。生物信息学分析结合筛选方法表明，dtdp -4-酮-6-脱氧-d-葡萄糖的外映体化是由两个旁系蛋白PP_1782 （RmlC1）和PP_0265 （RmlC2）催化的，而最终产物的还原是由PP_1784 （RmlD）单独介导的。因此，我们也排除了不同的RmlD同源物PP_0500和遗传关联的核苷二磷酸糖外基酶PP_0501参与dTDP-l-Rha形成的可能性，这与某些数据库的建议不同。总之，我们的分析有助于分子理解这种重要的核苷酸糖是如何在假单胞菌中合成的。
l-Rhamnose is an important monosaccharide both as nutrient source and as building block in prokaryotic glycoproteins and glycolipids. Generation of those composite molecules requires activated precursors being provided e. g. in form of nucleotide sugars such as dTDP-β-l-rhamnose (dTDP-l-Rha). dTDP-l-Rha is synthesized in a conserved 4-step reaction which is canonically catalyzed by the enzymes RmlABCD. An intact pathway is especially important for the fitness of pseudomonads, as dTDP-l-Rha is essential for the activation of the polyproline specific translation elongation factor EF-P in these bacteria. Within the scope of this study, we investigated the dTDP-l-Rha-biosynthesis route of Pseudomonas putida KT2440 with a focus on the last two steps. Bioinformatic analysis in combination with a screening approach revealed that epimerization of dTDP-4-keto-6-deoxy-d-glucose to dTDP-4-keto-6-deoxy-l-mannose is catalyzed by the two paralogous proteins PP_1782 (RmlC1) and PP_0265 (RmlC2), whereas the reduction to the final product is solely mediated by PP_1784 (RmlD). Thus, we also exclude the distinct RmlD homolog PP_0500 and the genetically linked nucleoside diphosphate-sugar epimerase PP_0501 to be involved in dTDP-l-Rha formation, other than suggested by certain databases. Together our analysis contributes to the molecular understanding how this important nucleotide-sugar is synthesized in pseudomonads.
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影响因子：
14.8
作者：
Kelley LA;Mezulis S;Yates CM;Wass MN;Sternberg MJ
通讯作者：
Sternberg MJ
DOI：
10.1107/s0907444999016200
发表时间：
2000-02-01
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2.2
作者：
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作者：
Giraud, MF;Naismith, JH
通讯作者：
Naismith, JH
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作者：
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通讯作者：
Choe, Jungwoo
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影响因子：
3.5
作者：
Gilreath MS;Roy H;Bullwinkle TJ;Katz A;Navarre WW;Ibba M
通讯作者：
Ibba M