Electrokinetic focusing and separation of mammalian cells in conductive biological fluids.
Electrokinetic focusing and separation of mammalian cells in conductive biological fluids.
复制标题
DOI：
10.1039/c2an35707k
复制
发表时间：
2012-11-21
期刊：
影响因子：
--
通讯作者：
Wong PK
中科院分区：
文献类型：
--
作者：
Gao J;Riahi R;Sin ML;Zhang S;Wong PK
细胞的主动操作，例如捕获、聚焦和分离，对于各种生物分析应用至关重要。在此，我们报告了一种用于在生理液体中操纵哺乳动物细胞的混合电动技术。该技术应用了负介电泳力和电流体动力学引起的流体动力拖曳力的组合，这在导电生物流体中是有效的。通过三电极配置，可以调整细胞的稳定平衡位置以用于分离和聚焦应用。在静态和动态流动条件下，癌细胞和白细胞都可以定位并分离到微通道中的特定位置。为了研究混合电动过程的灵敏度，系统地研究了细胞速度的交流电压、频率和偏置依赖性。通过连续聚焦尿液、血沉棕黄层和处理后的血液样本中的人乳腺腺癌，证明了混合电动技术在操纵生理样本中细胞的适用性，捕获效率为 98%。
Active manipulation of cells, such as trapping, focusing, and isolation, is essential for various bioanalytical applications. Herein, we report a hybrid electrokinetic technique for manipulating mammalian cells in physiological fluids. This technique applies a combination of negative dielectrophoretic force and hydrodynamic drag force induced by electrohydrodynamics, which is effective in conductive biological fluids. With a three-electrode configuration, the stable equilibrium positions of cells can be adjusted for separation and focusing applications. Cancer cells and white blood cells can be positioned and isolated into specific locations in the microchannel under both static and dynamic flow conditions. To investigate the sensitivity of the hybrid electrokinetic process, AC voltage, frequency, and bias dependences of the cell velocity were studied systematically. The applicability of the hybrid electrokinetic technique for manipulating cells in physiological samples is demonstrated by continuous focusing human breast adenocarcinoma spiked in urine, buffy coats, and processed blood samples with 98% capture efficiency.
登录
查看更多内容
影响因子：
14.8
作者：
Ogunniyi, Adebola O.;Story, Craig M.;Papa, Eliseo;Guillen, Eduardo;Love, J. Christopher
通讯作者：
Love, J. Christopher
影响因子：
4.7
作者：
Pratt ED;Huang C;Hawkins BG;Gleghorn JP;Kirby BJ
通讯作者：
Kirby BJ
影响因子：
6.1
作者：
Ng, Wee Yang;Goh, Shireen;Rodriguez, Isabel
通讯作者：
Rodriguez, Isabel
影响因子：
56.9
作者：
Huang, LR;Cox, EC;Sturm, JC
通讯作者：
Sturm, JC
DOI：
10.1158/1078-0432.ccr-10-1105
发表时间：
2010-10-15
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Lin HK;Zheng S;Williams AJ;Balic M;Groshen S;Scher HI;Fleisher M;Stadler W;Datar RH;Tai YC;Cote RJ
通讯作者：
Cote RJ