Developmental expression of four novel serine/threonine kinase receptors homologous to the activin/transforming growth factor‐β type II receptor family
Developmental expression of four novel serine/threonine kinase receptors homologous to the activin/transforming growth factor‐β type II receptor family
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与激活素/转化生长因子-β II 型受体家族同源的四种新型丝氨酸/苏氨酸激酶受体的发育表达
作者：
W. He;M. L. Gustafson;Seichii Hirobe;P. Donahoe
丝氨酸/苏氨酸激酶跨膜蛋白是一个新的生长因子信号转导家族，包括激活素II型受体和转化生长因子β的II型受体的几种亚型。为了克隆转化生长因子- β超家族成员缪勒管抑制物质的受体，利用从这些受体激酶结构域的保守区域设计的寡核苷酸引物，对胎鼠泌尿生殖嵴cDNA进行PCR扩增。我们以这种方式分离了四个新的受体（命名为R1-R4），每个受体都具有先前克隆的激酶的结构特征，包括一个小的细胞外配体结合域，一个单一的疏水跨膜域和一个细胞内丝氨酸/苏氨酸激酶域。此外，每一个都有独特的激酶亚结构域和保守的丝氨酸/苏氨酸激酶序列在这个家族中发现。Northern分析显示R1‐R4的mRNA表达在多个组织中，包括胎儿泌尿生殖嵴、睾丸、卵巢、脑和肺。原位杂交进一步将R1定位到14.5 ~ 15天胎鼠苗勒管间质以及前腔和前腔卵泡卵母细胞，这些位置与预测的苗勒管抑制物质受体定位一致。此外，R2特异定位于出生后睾丸的精小管。激活素和转化生长因子- β II型受体家族的这些最新成员应该有助于确定该配体超家族通过膜磷酸化影响细胞生长和分化的分子机制。©1993 wiley‐Liss, Inc。
Serine/threonine kinase transmembrane proteins are a new family of growth factor signal transducers that includes several isoforms of the activin type II receptor and the type II receptor for transforming growth factor‐β. In an effort to clone the receptor for Mullerian inhibiting substance, a member of the transforming growth factor‐β superfamily, oligonucleotide primers designed from conserved regions of these receptors' kinase domains were used for PCR amplification of fetal rat urogenital ridge cDNA. We isolated four novel receptors in this manner (desingnated R1–R4), each of which has structural features of the previously cloned kinases, including a small extracellular ligand‐binding domain, a single hydrophobic transmembrane domain, and an intracellular serine/threonine kinase domain. In addition, each has characteristic kinase subdomains and conserved serine/threonine kinase sequences found in this family. Northern analysis revealed mRNA expression of R1‐R4 in several tissues, including fetal urogenital ridge, testis, and ovary, as well as brain and lung. In situ hybridization further localized R1 to mesenchyme of the 14.5 to 15‐day fetal rat Mullerian duct and to oocytes of preantral and antral follicles, sites that are consistent with the predicted localization of Mullerian inhibiting substance receptor. In addition, R2 localized specifically to seminiferous tubules of the postnatal testis. These newest members of the activin and transforming growth factor‐β type II receptor family should help define the molecular mechanisms by which this ligand superfamily affects cell growth and differentiation via membrane phosphorylation. © 1993 wiley‐Liss, Inc.
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苗勒管抑制物质的蛋白水解加工产生转化生长因子-β样片段。
DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Pepinsky,RB;Sinclair,LK;Chow,EP;Mattaliano,RJ;Manganaro,TF;Donahoe,PK;Cate,RL
通讯作者：
Cate,RL
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影响因子：
4.8
作者：
Hirobe,S;He,WW;Lee,MM;Donahoe,PK
通讯作者：
Donahoe,PK
影响因子：
4.8
作者：
Shigeru Ueno;Tatsuo Kuroda;D. Maclaughlin;R. Ragin;T. Manganaro;P. K. Donahoe
通讯作者：
Shigeru Ueno;Tatsuo Kuroda;D. Maclaughlin;R. Ragin;T. Manganaro;P. K. Donahoe
人重组苗勒管抑制物质对大鼠苗勒管退化过程中分离的上皮和间质细胞的影响。
影响因子：
4.8
作者：
Tsuji,M;Shima,H;Yonemura,CY;Brody,J;Donahoe,PK;Cunha,GR
通讯作者：
Cunha,GR
苗勒氏管消退过程中的磷酸化事件。
DOI：
10.1126/science.6607531
发表时间：
1984
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Hutson,JM;Fallat,ME;Kamagata,S;Donahoe,PK;Budzik,GP
通讯作者：
Budzik,GP