HID-1 is a peripheral membrane protein primarily associated with the medial- and trans- Golgi apparatus
HID-1 is a peripheral membrane protein primarily associated with the medial- and trans- Golgi apparatus
复制标题
HID-1 是一种外周膜蛋白，主要与内侧和跨高尔基体相关
DOI：
10.1007/s13238-011-1008-3
复制
发表时间：
2011-02
期刊：
影响因子：
--
通讯作者：
L. Wang, Y. Zhan, E. Song, Y. Yu, Y. Jiu, W. Du, J
中科院分区：
文献类型：
--
作者：
T. Xu;L. Wang, Y. Zhan, E. Song, Y. Yu, Y. Jiu, W. Du, J
秀丽隐杆线虫HID-1基因是在筛选具有高温诱导达尔形成(HID)表型的突变株中首次发现的。尽管HID-1基因编码一种从秀丽线虫到哺乳动物高度保守的新蛋白(HID-1)，但HID-1的结构域结构、亚细胞定位和确切功能尚不清楚。以往的研究和各种生物信息学软件预测HID-1含有许多跨膜结构域，但没有已知的功能结构域。在这项研究中，我们揭示了哺乳动物HID-1定位于内侧和跨高尔基体以及胞浆，并且该定位对布雷菲尔丁A处理敏感。接下来，我们证明了HID-1是一种外周膜蛋白，在高尔基体和胞浆之间动态穿梭。最后，我们验证了HID-1与高尔基体结合需要一个保守的N末端肉豆蔻酰化位点。我们推测HID-1可能参与了高尔基体细胞内的转运。
Caenorhabditis elegans hid-1 gene was first identified in a screen for mutants with a high-temperature-induced dauer formation (Hid) phenotype. Despite the fact that the hid-1 gene encodes a novel protein (HID-1) which is highly conserved from Caenorhabditis elegans to mammals, the domain structure, subcellular localization, and exact function of HID-1 remain unknown. Previous studies and various bioinformatic softwares predicted that HID-1 contained many transmembrane domains but no known functional domain. In this study, we revealed that mammalian HID-1 localized to the medial- and trans- Golgi apparatus as well as the cytosol, and the localization was sensitive to brefeldin A treatment. Next, we demonstrated that HID-1 was a peripheral membrane protein and dynamically shuttled between the Golgi apparatus and the cytosol. Finally, we verified that a conserved N-terminal myristoylation site was required for HID-1 binding to the Golgi apparatus. We propose that HID-1 is probably involved in the intracellular trafficking within the Golgi region.
登录
查看更多内容
影响因子：
3.3
作者：
M. Ailion;James H. Thomas
通讯作者：
M. Ailion;James H. Thomas
影响因子：
3.3
作者：
Takao Inoué;James H. Thomas
通讯作者：
Takao Inoué;James H. Thomas
DOI：
10.1002/j.1460-2075.1986.tb04395.x
发表时间：
1986-07
期刊：
The EMBO Journal
影响因子：
--
作者：
M. Zerial;P. Melançon;C. Schneider;H. Garoff
通讯作者：
M. Zerial;P. Melançon;C. Schneider;H. Garoff
影响因子：
--
作者：
Danielle H. Dube;C. D. de Graffenried;Jennifer J. Kohler
通讯作者：
Danielle H. Dube;C. D. de Graffenried;Jennifer J. Kohler
影响因子：
3.1
作者：
Z. Liang;G. Li
通讯作者：
Z. Liang;G. Li