Fasciola gigantica: Ultrastructural cytochemistry of the tegumental surface in newly- excysted metacercariae and in vitro-penetrated juvenile flukes informs a concept of parasite defence at the interface with the host.
Fasciola gigantica: Ultrastructural cytochemistry of the tegumental surface in newly- excysted metacercariae and in vitro-penetrated juvenile flukes informs a concept of parasite defence at the interface with the host.
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巨大片形吸虫：新排出的囊蚴和体外穿透的幼年吸虫的皮膜表面的超微结构细胞化学揭示了寄生虫与宿主界面处的防御概念。
DOI：
10.1016/j.vetpar.2019.108923
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发表时间：
2019
影响因子：
2.6
通讯作者：
Hanna REB
中科院分区：
文献类型：
--
作者：
Hanna REB
采用细胞化学染色技术，在玻璃体切除和肠道穿透的巨片吸虫内进行细胞化学染色，以显示被膜的糖萼，这一结构包括宿主-寄生虫界面的寄生虫成分，但通过传统的固定和电子显微镜制备技术不能完全保存。Ru红和高碘酸-硫代肼-Os(PATCO)技术的阳性反应表明，糖萼是多阴离子的，在整个深度都富含碳水化合物。它包括一个三胺排列，有一个薄的致密区和与顶端质膜外部紧密相对的纤维层，包被一个不规则的厚粘多糖囊。后者在成体吸虫中没有记录，可能代表了一种特定的适应，以促进面对宿主免疫的入侵，也可能保护寄生虫表面免受宿主和寄生虫衍生的蛋白酶的作用。在幼稚宿主入侵的早期，糖萼可能部分地触发了天然免疫的反应，而在感染的后期，或者当具有免疫能力的宿主启动记忆反应时，特定获得性免疫的抗体和激活的淋巴细胞被调用来与寄生虫表面相互作用。糖萼的细胞化学性质，加上其由于T0被层分泌体的胞吐作用而动态周转的潜力，可能有助于中和潜在的破坏性免疫效应器，并通过与糖萼组分的复合体将它们从寄生虫附近清除。
Cytochemical staining techniques were carried outen blocwithin vitroexcysted and gut-penetratedFasciola giganticalarvae in order to visualise the glycocalyx of the tegument, a structure which comprises the parasite component of the host-parasite interface, yet is incompletely preserved by conventional fixation and preparation techniques for electron microscopy. Positive reactivity with ruthenium red and periodic acid-thiocarbohydrazine-osmium (PATCO) techniques revealed that the glycocalyx is polyanionic and carbohydrate-rich throughout its depth. It comprises a trilaminate arrangement, with a thin dense zone and fibrillar layer closely apposed to the outer aspect of the apical plasma membrane, invested by an irregular thick mucopolysaccharide capsule. The latter, not recorded in adult flukes, may represent a specific adaptation to facilitate invasion in the face of host immunity, and may also protect the parasite surface from the action of host- and parasite-derived proteases. Early in the invasion of a naïve host, the glycocalyx may be partly responsible for triggering the responses of innate immunity, while later in infection, or when an anamnestic response is initiated in an immunocompetent host, the antibodies and activated lymphocytes of specific acquired immunity are invoked to interact with the parasite surface. The cytochemical properties of the glycocalyx, together with its potential for dynamic turnover due to exocytosis of the T0 tegumental secretory bodies, are likely to aid neutralisation of potentially damaging immune effectors and ensure their removal from the vicinity of the parasite by sloughing in complex with glycocalyx components.
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影响因子：
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DOI：
--
发表时间：
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期刊：
影响因子：
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