Construction and characterisation of a heparan sulphate heptasaccharide microarray.

Construction and characterisation of a heparan sulphate heptasaccharide microarray.
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DOI:
10.1039/c6cc08204a
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发表时间:
2017-01-31
期刊:
Chemical communications (Cambridge, England)
影响因子:
--
通讯作者:
Liu J
Liu J
中科院分区:
其他
文献类型:
--
作者:
Yang J;Hsieh PH;Liu X;Zhou W;Zhang X;Zhao J;Xu Y;Zhang F;Linhardt RJ;Liu J

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合成靶向七糖库以制备硫酸乙酰肝素(HS)微阵列。该阵列探测与两个聚糖结合蛋白,HS 3-O-磺基转移酶1和抗凝血酶,证明HS和蛋白质之间的结合选择性。HS基因芯片技术将有助于更好地了解HS的结构和功能关系。
A targeted heptasaccharide library was synthesised to prepare a heparan sulphate (HS) microarray. The array was probed with two glycan-binding proteins, HS 3-O-sulphotransferase 1 and antithrombin, demonstrating the binding selectivity between HS and proteins. The HS microarray technique will accelerate the understanding of the structure and function relationships of HS.
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