Modulation of flavonoid metabolites in Arabidopsis thaliana through overexpression of the MYB75 transcription factor: role of kaempferol-3,7-dirhamnoside in resistance to the specialist insect herbivore Pieris brassicae.
Modulation of flavonoid metabolites in Arabidopsis thaliana through overexpression of the MYB75 transcription factor: role of kaempferol-3,7-dirhamnoside in resistance to the specialist insect herbivore Pieris brassicae.
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DOI：
10.1093/jxb/eru096
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发表时间：
2014-05
影响因子：
6.9
通讯作者：
Dicke M
中科院分区：
文献类型：
--
作者：
Onkokesung N;Reichelt M;van Doorn A;Schuurink RC;van Loon JJ;Dicke M
MYB 75转录因子是花青素苷生物合成的调节因子。过度表达MYB 75导致槲皮素/山奈酚代谢物的重新通道化，包括山奈酚-3，7-二鼠李糖苷的积累减少，山奈酚-3，7-二鼠李糖苷是一种针对专业毛虫的新型防御代谢物。花色素苷和黄酮醇是植物的次生代谢产物，在植物防御中具有重要作用。在拟南芥中，花青苷和黄酮醇的生物合成受MYB转录因子调控。MYB 75（oxMYB 75）在拟南芥中的过表达导致花青素和黄酮醇水平增加，这增强了植物对多面手毛虫的抗性。然而，这些代谢物如何影响专业食草动物仍然是未知的。在oxMYB 75植物上喂养后，专家蚜虫（Brassicae短角蚜）的性能不受影响，而专家毛虫（Pieris brassicae）在这种植物上喂养时获得了显着更高的体重。花青素和总黄酮醇糖苷水平的增加呈负相关的体重喂oxMYB 75植物的毛虫。然而，山奈酚-3，7-二鼠李糖苷（KRR）的显著减少对应于oxMYB 75植物对毛虫摄食的敏感性增加。菜粉蝶毛虫在含有KRR的人工饲料或用KRR外源处理的oxMYB 75植物上也生长较少，支持KRR在直接防御这种专业毛虫方面的功能。结果表明，增强oxMYB 75植物中花青素途径的活性导致槲皮素/山奈酚代谢物的重新引导，这对KRR的积累具有负面影响，KRR是一种针对专业毛虫的新型防御代谢物。
The MYB75 transcription factor is a regulator of anthocyanin biosynthesis. Overexpressing MYB75 results in re-channelling of quercetin/kaempferol metabolites including reduced accumulation of kaempferol-3,7-dirhamnoside, a novel defensive metabolite against a specialist caterpillar. Anthocyanins and flavonols are secondary metabolites that can function in plant defence against herbivores. In Arabidopsis thaliana, anthocyanin and flavonol biosynthesis are regulated by MYB transcription factors. Overexpression of MYB75 (oxMYB75) in Arabidopsis results in increasing anthocyanin and flavonol levels which enhances plant resistance to generalist caterpillars. However, how these metabolites affect specialist herbivores has remained unknown. Performance of a specialist aphid (Brevicoryne brassicae) was unaffected after feeding on oxMYB75 plants, whereas a specialist caterpillar (Pieris brassicae) gained significantly higher body mass when feeding on this plant. An increase in anthocyanin and total flavonol glycoside levels correlated negatively with the body mass of caterpillars fed on oxMYB75 plants. However, a significant reduction of kaempferol-3,7-dirhamnoside (KRR) corresponded to an increased susceptibility of oxMYB75 plants to caterpillar feeding. Pieris brassicae caterpillars also grew less on an artificial diet containing KRR or on oxMYB75 plants that were exogenously treated with KRR, supporting KRR’s function in direct defence against this specialist caterpillar. The results show that enhancing the activity of the anthocyanin pathway in oxMYB75 plants results in re-channelling of quercetin/kaempferol metabolites which has a negative effect on the accumulation of KRR, a novel defensive metabolite against a specialist caterpillar.
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