Rapid detection and differentiation of swine-origin influenza A virus (H1N1/2009) from other seasonal influenza A viruses.
Rapid detection and differentiation of swine-origin influenza A virus (H1N1/2009) from other seasonal influenza A viruses.
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DOI：
10.3390/v4113012
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发表时间：
2012-11-09
期刊：
影响因子：
--
通讯作者：
Hewlett I
中科院分区：
文献类型：
--
作者：
Zhao J;Wang X;Ragupathy V;Zhang P;Tang W;Ye Z;Eichelberger M;Hewlett I
我们以前开发了一种快速和简单的金纳米颗粒（NP）为基础的基因组微阵列检测禽流感病毒H5 N1和其他流感病毒株（H1N1，H3 N2）的鉴别。在这项研究中，我们扩展了平台，以检测2009年猪源性甲型流感病毒（H1N1/2009）。针对H1N1/2009病毒的基质（M）、血凝素（HA）和神经氨酸酶（NA）基因设计了多种特异性捕获和中间寡核苷酸。以与季节性流感H1N1和H3 N2的HA、NA和M基因相同的格式打印H1N1/2009微阵列。使用捕获-靶-中间寡核苷酸杂交和金NP介导的银染色检测病毒RNA。来自HA和NA基因的4个捕获-靶-中间体的信号对H1N1/2009病毒具有特异性，并且未显示与来自其他流感毒株H1N1、H3 N2和H5 N1的病毒RNA的交叉杂交。所有的3 M基因捕获物显示出与H1N1/2009病毒RNA的强亲和力，其中2个3 M基因捕获物显示出与H1N1、H3 N2和H5 N1测试样品的交叉杂交。目前的检测方法能够检测H1N1/2009，并将其与其他甲型流感病毒区分开来。这种新方法可能有助于同时检测和分型甲型流感病毒，并可迅速修改，以检测其他新出现的流感病毒株在公共卫生环境。
We previously developed a rapid and simple gold nanoparticle(NP)-based genomic microarray assay for identification of the avian H5N1 virus and its discrimination from other influenza A virus strains (H1N1, H3N2). In this study, we expanded the platform to detect the 2009 swine-origin influenza A virus (H1N1/2009). Multiple specific capture and intermediate oligonucleotides were designed for the matrix (M), hemagglutinin (HA), and neuraminidase (NA) genes of the H1N1/2009 virus. The H1N1/2009 microarrays were printed in the same format as those of the seasonal influenza H1N1 and H3N2 for the HA, NA, and M genes. Viral RNA was tested using capture-target-intermediate oligonucleotide hybridization and gold NP-mediated silver staining. The signal from the 4 capture-target-intermediates of the HA and NA genes was specific for H1N1/2009 virus and showed no cross hybridization with viral RNA from other influenza strains H1N1, H3N2, and H5N1. All of the 3 M gene captures showed strong affinity with H1N1/2009 viral RNA, with 2 out of the 3 M gene captures showing cross hybridization with the H1N1, H3N2, and H5N1 samples tested. The current assay was able to detect H1N1/2009 and distinguish it from other influenza A viruses. This new method may be useful for simultaneous detection and subtyping of influenza A viruses and can be rapidly modified to detect other emerging influenza strains in public health settings.
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影响因子：
5.2
作者：
Briand S;Mounts A;Chamberland M
通讯作者：
Chamberland M
影响因子：
3.5
作者：
Zhao J;Tang S;Storhoff J;Marla S;Bao YP;Wang X;Wong EY;Ragupathy V;Ye Z;Hewlett IK
通讯作者：
Hewlett IK
DOI：
10.1126/science.1189132
发表时间：
2010-06-18
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Vijaykrishna D;Poon LL;Zhu HC;Ma SK;Li OT;Cheung CL;Smith GJ;Peiris JS;Guan Y
通讯作者：
Guan Y
DOI：
10.1073/pnas.0507415102
发表时间：
2005-12-20
影响因子：
11.1
作者：
Gabriel, G;Dauber, B;Stech, J
通讯作者：
Stech, J
影响因子：
3.7
作者：
Rohm, C;Zhou, NA;Webster, RG
通讯作者：
Webster, RG