Dephosphorylation of F-BAR protein Cdc15 modulates its conformation and stimulates its scaffolding activity at the cell division site.
Dephosphorylation of F-BAR protein Cdc15 modulates its conformation and stimulates its scaffolding activity at the cell division site.
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DOI：
10.1016/j.molcel.2010.06.012
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发表时间：
2010-07-09
期刊：
影响因子：
16
通讯作者：
Gould KL
中科院分区：
文献类型：
--
作者：
Roberts-Galbraith RH;Ohi MD;Ballif BA;Chen JS;McLeod I;McDonald WH;Gygi SP;Yates JR 3rd;Gould KL
裂殖酵母的胞质分裂需要CDC15的功能，CDC15是Pombe cdc15同源蛋白家族的创始成员。作为一种早期的、丰富的具有多个结合伙伴的收缩环成分，CDC15在环的组织中起着关键的作用。我们证明了在许多位置的CDC15磷酸化产生一个封闭的构象，在间期抑制了CDC15在分裂位置的组装，并阻止了CDC15与其结合伙伴的相互作用。在有丝分裂过程中，CDC15去磷酸化诱导开放的构象、寡聚和支架活性。磷酸化水平降低的CDC15突变体早熟地出现在丝状结构的分裂部位，并与蛋白伴侣和膜结合增加。我们的结果表明，CDC15的磷酸调节促进了收缩装置的组装和拆解，并提示了PCH家族和BAR超家族成员可能广泛采用的调控策略，以实现其作为膜和细胞骨架之间的连接物的时间特异性。
Cytokinesis in Schizosaccharomyces pombe requires the function of Cdc15, founding member of the pombe cdc15 homology (PCH) family of proteins. As an early, abundant contractile ring component with multiple binding partners, Cdc15 plays a key role in organizing the ring. We demonstrate that Cdc15 phosphorylation at many sites generates a closed conformation, inhibits Cdc15 assembly at the division site in interphase, and precludes interaction of Cdc15 with its binding partners. Cdc15 dephosphorylation induces an open conformation, oligomerization, and scaffolding activity during mitosis. Cdc15 mutants with reduced phosphorylation precociously appear at the division site in filament-like structures and display increased association with protein partners and the membrane. Our results indicate that Cdc15 phosphoregulation impels both assembly and disassembly of the contractile apparatus and suggest a regulatory strategy that PCH family and BAR superfamily members might broadly employ to achieve temporal specificity in their roles as linkers between membrane and cytoskeleton.
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芽殖酵母酿酒酵母中内体和肌动蛋白斑块的组装、拆卸和肌动蛋白电缆依赖性运动的活细胞成像。
影响因子：
7.8
作者：
Huckaba, TM;Gay, AC;Pantalena, LF;Yang, HC;Pon, LA
通讯作者：
Pon, LA
DOI：
10.1083/jcb.200402097
发表时间：
2004-06-07
期刊：
The Journal of cell biology
影响因子：
--
作者：
Motegi F;Mishra M;Balasubramanian MK;Mabuchi I
通讯作者：
Mabuchi I
影响因子：
4.8
作者：
Bauerfeind, R;Takei, K;De Camilli, P
通讯作者：
De Camilli, P
DOI：
10.1073/pnas.0607084104
发表时间：
2007-02-13
影响因子：
11.1
作者：
Chi, An;Huttenhower, Curtis;Hunt, Donald F.
通讯作者：
Hunt, Donald F.
影响因子：
5.8
作者：
Dosztányi, Z;Csizmok, V;Simon, I
通讯作者：
Simon, I