Non-host Resistance Induced by the Xanthomonas Effector XopQ Is Widespread within the Genus Nicotiana and Functionally Depends on EDS1.
Non-host Resistance Induced by the Xanthomonas Effector XopQ Is Widespread within the Genus Nicotiana and Functionally Depends on EDS1.
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DOI：
10.3389/fpls.2016.01796
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发表时间：
2016
影响因子：
5.6
通讯作者：
Bonas U
中科院分区：
文献类型：
--
作者：
Adlung N;Prochaska H;Thieme S;Banik A;Blüher D;John P;Nagel O;Schulze S;Gantner J;Delker C;Stuttmann J;Bonas U
大多数革兰氏阴性植物病原菌通过保守的 III 型分泌系统将效应蛋白 (T3E) 直接转移到植物细胞中，这对于易感植物的致病性至关重要。在抗性植物中，一些 T3E 的识别是由相应的抗性 (R) 基因或 R 蛋白介导的，并诱导效应触发免疫 (ETI)，这通常会导致程序性细胞死亡反应。 R 基因的鉴定并了解其进化/分布对于产生抗性作物具有巨大潜力。我们专注于来自 Xanthomonas Campestris pv. 的 T3E。 vesicatoria (Xcv)，辣椒和番茄植物上细菌性斑点病的病原体。在这里，主要是烟草属的 86 个茄科品系在根癌农杆菌介导的 21 个不同 Xcv 效应子瞬时表达后进行表型反应筛选，以 (i) 鉴定用于 T3E 表征的新植物品系，(ii) 分析假定 R 基因的保守/进化，以及 (iii) 鉴定有前途的植物品系作为 R 基因分离的库。效应子在密切相关的植物品系上引起不同的反应，表明潜在 R 基因的高变异性和进化率。在某些情况下，假定的 R 基因在植物物种内保守，但在上位系统发育单位内不保守。有趣的是，效应子 XopQ 被几种烟草属植物识别。品系和 Xcv 感染测定表明，XopQ 是许多烟草属物种的宿主范围决定因素。本塞姆氏烟草中针对 Xcv 和 XopQ 识别的非宿主抗性需要 EDS1，强烈表明这些植物品系中存在包含 TIR 结构域的 XopQ 特异性 R 蛋白。 XopQ 是大多数黄单胞菌中的保守效应子，指出识别 XopQ 的 RxopQ 是有针对性的作物改良的候选者。
Most Gram-negative plant pathogenic bacteria translocate effector proteins (T3Es) directly into plant cells via a conserved type III secretion system, which is essential for pathogenicity in susceptible plants. In resistant plants, recognition of some T3Es is mediated by corresponding resistance (R) genes or R proteins and induces effector triggered immunity (ETI) that often results in programmed cell death reactions. The identification of R genes and understanding their evolution/distribution bears great potential for the generation of resistant crop plants. We focus on T3Es from Xanthomonas campestris pv. vesicatoria (Xcv), the causal agent of bacterial spot disease on pepper and tomato plants. Here, 86 Solanaceae lines mainly of the genus Nicotiana were screened for phenotypical reactions after Agrobacterium tumefaciens-mediated transient expression of 21 different Xcv effectors to (i) identify new plant lines for T3E characterization, (ii) analyze conservation/evolution of putative R genes and (iii) identify promising plant lines as repertoire for R gene isolation. The effectors provoked different reactions on closely related plant lines indicative of a high variability and evolution rate of potential R genes. In some cases, putative R genes were conserved within a plant species but not within superordinate phylogenetical units. Interestingly, the effector XopQ was recognized by several Nicotiana spp. lines, and Xcv infection assays revealed that XopQ is a host range determinant in many Nicotiana species. Non-host resistance against Xcv and XopQ recognition in N. benthamiana required EDS1, strongly suggesting the presence of a TIR domain-containing XopQ-specific R protein in these plant lines. XopQ is a conserved effector among most xanthomonads, pointing out the XopQ-recognizing RxopQ as candidate for targeted crop improvement.
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DOI：
10.1111/nph.12214
发表时间：
2013-09
期刊：
The New phytologist
影响因子：
--
作者：
Henry E;Yadeta KA;Coaker G
通讯作者：
Coaker G
影响因子：
3.7
作者：
Engler, Carola;Kandzia, Romy;Marillonnet, Sylvestre
通讯作者：
Marillonnet, Sylvestre
影响因子：
7.4
作者：
Buettner, Daniela;He, Sheng Yang
通讯作者：
He, Sheng Yang
影响因子：
3.5
作者：
BONAS, U;SCHULTE, R;STALL, RE
通讯作者：
STALL, RE
影响因子：
4.2
作者：
Chase, MW;Knapp, S;Parokonny, AS
通讯作者：
Parokonny, AS