Bomidin: An Optimized Antimicrobial Peptide With Broad Antiviral Activity Against Enveloped Viruses.
Bomidin: An Optimized Antimicrobial Peptide With Broad Antiviral Activity Against Enveloped Viruses.
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DOI：
10.3389/fimmu.2022.851642
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发表时间：
2022
影响因子：
7.3
通讯作者：
中科院分区：
文献类型：
--
作者：
高传染性病原体的快速演变是对全球公共卫生的重大威胁。在抵御细菌、真菌和病毒的第一线，抗菌肽(AMPs)是由所有生物自然产生的，为下一代抗生素的开发提供了新的可能性。然而，AMPS的产率低，提取和纯化困难，阻碍了其工业和科学研究的应用。为了克服这些障碍，我们实现了Bomidin的高效表达，Bomidin是一种基于牛髓系抗菌肽-27的商业重组AMP。这种新型的AMP可以通过在Bomidin序列中添加蛋氨酸在大肠杆菌中表达，可以批量生产，并且比化学合成的AMP具有更高的生物活性。我们验证了blomidin对多种细菌和包膜病毒的作用，包括严重急性呼吸综合征冠状病毒2(SARS-CoV-2)、单纯疱疹病毒(HSV)、登革病毒(DENV)和基孔肯雅病毒(CHIKV)。此外，我们还基于Bomidin和膜脂的分子模型，阐明了Bomidin破坏细菌和病毒细胞膜的可能机制。因此，我们获得了一种新型的AMP，它具有优化的、高效的异源表达系统，可用于治疗广泛威胁生命的病原体。
The rapid evolution of highly infectious pathogens is a major threat to global public health. In the front line of defense against bacteria, fungi, and viruses, antimicrobial peptides (AMPs) are naturally produced by all living organisms and offer new possibilities for next-generation antibiotic development. However, the low yields and difficulties in the extraction and purification of AMPs have hindered their industry and scientific research applications. To overcome these barriers, we enabled high expression of bomidin, a commercial recombinant AMP based upon bovine myeloid antimicrobial peptide-27. This novel AMP, which can be expressed in Escherichia coli by adding methionine to the bomidin sequence, can be produced in bulk and is more biologically active than chemically synthesized AMPs. We verified the function of bomidin against a variety of bacteria and enveloped viruses, including severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), herpes simplex virus (HSV), dengue virus (DENV), and chikungunya virus (CHIKV). Furthermore, based on the molecular modeling of bomidin and membrane lipids, we elucidated the possible mechanism by which bomidin disrupts bacterial and viral membranes. Thus, we obtained a novel AMP with an optimized, efficient heterologous expression system for potential therapeutic application against a wide range of life-threatening pathogens.
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影响因子：
5.5
作者：
Hess, Berk;Kutzner, Carsten;Lindahl, Erik
通讯作者：
Lindahl, Erik
包膜病毒的肽进入抑制剂：界面疏水性的重要性。
DOI：
10.1016/j.bbamem.2014.04.015
发表时间：
2014-09
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Badani H;Garry RF;Wimley WC
通讯作者：
Wimley WC
影响因子：
64.8
作者：
Bhardwaj, Gaurav;Mulligan, Vikram Khipple;Bahl, Christopher D.;Gilmore, Jason M.;Harvey, Peta J.;Cheneval, Olivier;Buchko, Garry W.;Pulavarti, Surya V. S. R. K.;Kaas, Quentin;Eletsky, Alexander;Huang, Po-Ssu;Johnsen, William A.;Greisen, Per Jr;Rocklin, Gabriel J.;Song, Yifan;Linsky, Thomas W.;Watkins, Andrew;Rettie, Stephen A.;Xu, Xianzhong;Carter, Lauren P.;Bonneau, Richard;Olson, James M.;Coutsias, Evangelos;Correnti, Colin E.;Szyperski, Thomas;Craik, David J.;Baker, David
通讯作者：
Baker, David
DOI：
10.1371/currents.outbreaks.0411252a8b82aa933f6540abb54a855f
发表时间：
2014-09-02
期刊：
PLoS currents
影响因子：
--
作者：
Afreen, Nazia;Deeba, Farah;Parveen, Shama
通讯作者：
Parveen, Shama
影响因子：
4.1
作者：
Brice, David C.;Diamond, Gill
通讯作者：
Diamond, Gill