Transcriptome of American oysters, Crassostrea virginica, in response to bacterial challenge: insights into potential mechanisms of disease resistance.
Transcriptome of American oysters, Crassostrea virginica, in response to bacterial challenge: insights into potential mechanisms of disease resistance.
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DOI：
10.1371/journal.pone.0105097
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Gomez-Chiarri M
中科院分区：
文献类型：
--
作者：
McDowell IC;Nikapitiya C;Aguiar D;Lane CE;Istrail S;Gomez-Chiarri M
美国牡蛎Crassostrea virginica是一种重要的生态和经济河口生物，在美国东北部地区由于由革兰氏阴性细菌病原体Roseovarius crassostreae引起的Roseovarius牡蛎病（ROD）而死亡率很高。本研究的目的是：1）美国牡蛎对R。crassostrae，和2）潜在的机制，电阻或敏感性ROD。牡蛎对细菌挑战的反应是通过将来自ROD抗性和敏感家族的牡蛎暴露于R. crassostrae，然后对来自疾病攻击后不同时间点的cDNA样品进行高通量测序。序列数据被组装成一个参考转录组，并通过差异基因表达和功能富集进行分析，以揭示可能参与美国牡蛎对ROD反应的基因和过程。而敏感牡蛎在受到R. crassostreae抗性牡蛎显示出与未受攻击的牡蛎相似的死亡率水平。牡蛎暴露于R. crassostreae显示出参与免疫识别、信号传导、蛋白酶抑制、解毒和凋亡的转录物的差异表达。参与代谢的转录本在易感牡蛎中富集，表明细菌感染对这些牡蛎有很大的代谢需求。在抵抗感染的牡蛎中差异表达的转录本包括免疫调节剂IL-17和β-内酰胺酶，以及参与细胞外基质重塑的几个基因。目前，对参与防御红曲霉的基因和过程的研究还处于起步阶段。美国牡蛎中的crassostrae提供了对抗病潜在机制的见解。
The American oyster Crassostrea virginica, an ecologically and economically important estuarine organism, can suffer high mortalities in areas in the Northeast United States due to Roseovarius Oyster Disease (ROD), caused by the gram-negative bacterial pathogen Roseovarius crassostreae. The goals of this research were to provide insights into: 1) the responses of American oysters to R. crassostreae, and 2) potential mechanisms of resistance or susceptibility to ROD. The responses of oysters to bacterial challenge were characterized by exposing oysters from ROD-resistant and susceptible families to R. crassostreae, followed by high-throughput sequencing of cDNA samples from various timepoints after disease challenge. Sequence data was assembled into a reference transcriptome and analyzed through differential gene expression and functional enrichment to uncover genes and processes potentially involved in responses to ROD in the American oyster. While susceptible oysters experienced constant levels of mortality when challenged with R. crassostreae, resistant oysters showed levels of mortality similar to non-challenged oysters. Oysters exposed to R. crassostreae showed differential expression of transcripts involved in immune recognition, signaling, protease inhibition, detoxification, and apoptosis. Transcripts involved in metabolism were enriched in susceptible oysters, suggesting that bacterial infection places a large metabolic demand on these oysters. Transcripts differentially expressed in resistant oysters in response to infection included the immune modulators IL-17 and arginase, as well as several genes involved in extracellular matrix remodeling. The identification of potential genes and processes responsible for defense against R. crassostreae in the American oyster provides insights into potential mechanisms of disease resistance.
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通过数字基因表达分析，成功免疫反应在牡蛎crassostrea gigas中成功免疫反应的整体转录组分析。
影响因子：
3.7
作者：
de Lorgeril J;Zenagui R;Rosa RD;Piquemal D;Bachère E
通讯作者：
Bachère E
影响因子：
3.4
作者：
Boardman, Cynthia L.;Maloy, Aaron P.;Boettcher, Katherine J.
通讯作者：
Boettcher, Katherine J.
DOI：
10.1152/ajpheart.1998.274.1.h342
发表时间：
1998-01-01
影响因子：
4.8
作者：
Chang, CI;Liao, JC;Kuo, L
通讯作者：
Kuo, L
DOI：
10.1007/978-90-481-8639-6_7
发表时间：
2010-01-01
期刊：
INTRODUCTION TO MARINE GENOMICS
影响因子：
--
作者：
Cancela, M. Leonor;Bargelloni, Luca;Volckaert, Filip A. M.
通讯作者：
Volckaert, Filip A. M.
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y