Calibration-Free Measurement of Phenylalanine Levels in the Blood Using an Electrochemical Aptamer-Based Sensor Suitable for Point-of-Care Applications.
Calibration-Free Measurement of Phenylalanine Levels in the Blood Using an Electrochemical Aptamer-Based Sensor Suitable for Point-of-Care Applications.
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DOI：
10.1021/acssensors.9b01703
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发表时间：
2019-12-27
期刊：
影响因子：
8.9
通讯作者：
Plaxco KW
中科院分区：
文献类型：
--
作者：
Idili A;Parolo C;Ortega G;Plaxco KW
通过类比在糖尿病治疗中创造的“家用葡萄糖监测器”的革命，模块化“平台”技术的可用性能够在未处理的、手指针刺量的全血中“在家”测量几乎任何代谢物、生物标志物或药物，这可能会彻底改变疾病的监测和治疗。因此，我们在此采用了基于电化学适体的传感平台来快速方便地测量血液中苯丙氨酸的水平，这是一种有助于监测和管理苯丙酮尿症（PKU）的能力。为了实现这一点，我们利用了先前报道的DNA适体，其识别与基于铑的“受体”复合的苯丙氨酸，从而提高亲和力。我们重新设计了它，使其在用亚甲基蓝氧化还原报告基因修饰它并将其连接到支持适当电化学询问的金电极之前经历大规模的结合诱导的构象变化。所得传感器实现了90 nM至7 μM的有效动态范围。当使用手指针刺标度的全血样本体积（稀释1000倍以匹配传感器的动态范围）进行测试时，该设备可在几分钟内实现血液苯丙氨酸水平的精确（±20%）、免校准测量。
By analogy to the revolution the “home glucose monitor” created in the treatment of diabetes, the availability of a modular, “platform” technology able to measure nearly any metabolite, biomarker, or drug “at-home” in unprocessed, finger-prick volumes of whole blood could revolutionize the monitoring and treatment of disease. Thus motivated, we have adapted here the electrochemical aptamer-based sensing platform to the problem of rapidly and conveniently measuring the level of phenylalanine in the blood, an ability that would aid the monitoring and management of phenylketonuria (PKU). To achieve this, we exploited a previously reported DNA aptamer that recognizes phenylalanine in complex with a rhodium-based “receptor” that improves affinity. We re-engineered this to undergo a large-scale, binding-induced conformational change before modifying it with a methylene blue redox reporter and attaching it to a gold electrode that supports the appropriate electrochemical interrogation. The resultant sensor achieves a useful dynamic range of 90 nM to 7 μM. When challenged with finger-prick-scale sample volumes of the whole blood (diluted 1000-fold to match the sensor’s dynamic range), the device achieves the accurate (±20%), calibration-free measurement of blood phenylalanine levels in minutes.
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影响因子：
7.4
作者：
Idili, Andrea;Amodio, Alessia;Vidonis, Marco;Feinberg-Somerson, Jacob;Castronovo, Matteo;Ricci, Francesco
通讯作者：
Ricci, Francesco
影响因子：
8.4
作者：
Idili, Andrea;Arroyo-Curras, Netzahualcoyotl;Plaxco, Kevin W.
通讯作者：
Plaxco, Kevin W.
影响因子：
15
作者：
Li H;Dauphin-Ducharme P;Ortega G;Plaxco KW
通讯作者：
Plaxco KW
DOI：
10.3390/molecules23040912
发表时间：
2018-04-15
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Somerson J;Plaxco KW
通讯作者：
Plaxco KW
影响因子：
2
作者：
Morrin, A;Killard, AJ;Smyth, MR
通讯作者：
Smyth, MR