The Saccharomyces cerevisiae MYO2 gene encodes an essential myosin for vectorial transport of vesicles.
The Saccharomyces cerevisiae MYO2 gene encodes an essential myosin for vectorial transport of vesicles.
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DOI：
10.1083/jcb.113.3.539
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发表时间：
1991-05
期刊：
影响因子：
--
通讯作者：
Singer RA
中科院分区：
文献类型：
--
作者：
Johnston GC;Prendergast JA;Singer RA
芽形成开始后，酿酒酵母的细胞将新的生长引导到发育中的芽。我们在这里表明，这种载体的增长是促进MYO 2基因的活性。野生型MYO 2基因编码肌球蛋白的基本形式，由其他肌球蛋白的球状肌动蛋白结合结构域的典型NH 2末端结构域组成。该NH 2-末端结构域通过似乎是短α-螺旋结构域连接到新的COOH-末端区域。在限制性温度下，myo 2 -66突变不会损害DNA、RNA或蛋白质的生物合成活性，但会产生未出芽的、扩大的细胞。这种表型表明细胞生长的定位缺陷。细胞大小的测量表明，启动芽的继续发展，以及芽的起始本身，受到抑制。大量分泌在突变体细胞中继续，尽管分泌囊泡积累。因此MYO 2肌球蛋白可能作为分子马达将分泌囊泡沿沿着肌动蛋白索运输到芽发育的位点。
After the initiation of bud formation, cells of the yeast Saccharomyces cerevisiae direct new growth to the developing bud. We show here that this vectorial growth is facilitated by activity of the MYO2 gene. The wild-type MYO2 gene encodes an essential form of myosin composed of an NH2-terminal domain typical of the globular, actin-binding domain of other myosins. This NH2-terminal domain is linked by what appears to be a short alpha-helical domain to a novel COOH-terminal region. At the restrictive temperature the myo2-66 mutation does not impair DNA, RNA, or protein biosynthetic activity, but produces unbudded, enlarged cells. This phenotype suggests a defect in localization of cell growth. Measurements of cell size demonstrated that the continued development of initiated buds, as well as bud initiation itself, is inhibited. Bulk secretion continues in mutant cells, although secretory vesicles accumulate. The MYO2 myosin thus may function as the molecular motor to transport secretory vesicles along actin cables to the site of bud development.
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