Estimates of total neuron number show that neonatal ethanol causes immediate and lasting neuron loss in cortical and subcortical areas.
Estimates of total neuron number show that neonatal ethanol causes immediate and lasting neuron loss in cortical and subcortical areas.
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DOI：
10.3389/fnins.2023.1186529
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发表时间：
2023
影响因子：
4.3
通讯作者：
Saito, Mariko
中科院分区：
文献类型：
--
作者：
Smiley, John F. F.;Bleiwas, Cynthia;Marino, Brandon M. M.;Vaddi, Prerana;Canals-Baker, Stefanie;Wilson, Donald A. A.;Saito, Mariko
关键词：
在新生儿脑发育过程中，有一段正常的凋亡细胞死亡期，调节成体神经元的数量。大约在同一时期，乙醇暴露可引起凋亡细胞死亡的急剧增加。虽然乙醇诱导的细胞凋亡已被证明可以减少成年神经元的数量，但关于乙醇效应的区域选择性以及大脑是否有能力克服最初的神经元损失的问题仍然存在。本研究使用立体学细胞计数来比较出生后第7天（P7）乙醇处理后8小时（P70）的累积神经元损失（P70）。在几个脑区，我们发现8小时后神经元总数的减少与成年动物一样大。不同区域间的比较显示，部分区域的神经元丢失较为脆弱，主要表现为丘脑前核>、中隔/垂直对角带、背下带、背外侧膝状核>、乳状体、扣带皮层>、整个新皮层的神经元丢失。与估计总神经元数量相比，乙醇处理后8小时nissl染色切片中凋亡细胞数量的估计提供了一个不太可靠的成年神经元损失预测指标。研究结果表明，乙醇诱导的新生儿细胞凋亡通常会导致成年后持续存在的即时神经元缺陷，并且进一步表明大脑可能具有有限的能力来补偿乙醇诱导的神经元损失。
In neonatal brain development there is a period of normal apoptotic cell death that regulates adult neuron number. At approximately the same period, ethanol exposure can cause a dramatic spike in apoptotic cell death. While ethanol-induced apoptosis has been shown to reduce adult neuron number, questions remain about the regional selectivity of the ethanol effect, and whether the brain might have some capacity to overcome the initial neuron loss. The present study used stereological cell counting to compare cumulative neuron loss 8 h after postnatal day 7 (P7) ethanol treatment to that of animals left to mature to adulthood (P70). Across several brain regions we found that the reduction of total neuron number after 8 h was as large as that of adult animals. Comparison between regions revealed that some areas are more vulnerable, with neuron loss in the anterior thalamic nuclei > the medial septum/vertical diagonal band, dorsal subiculum, and dorsal lateral geniculate nucleus > the mammillary bodies and cingulate cortex > whole neocortex. In contrast to estimates of total neuron number, estimates of apoptotic cell number in Nissl-stained sections at 8 h after ethanol treatment provided a less reliable predictor of adult neuron loss. The findings show that ethanol-induced neonatal apoptosis often causes immediate neuron deficits that persist in adulthood, and furthermore suggests that the brain may have limited capacity to compensate for ethanol-induced neuron loss.
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影响因子：
2.5
作者：
Ahern, Todd H.;Krug, Stefanie;Forger, Nancy G.
通讯作者：
Forger, Nancy G.
DOI：
10.1111/j.1530-0277.1993.tb00808.x
发表时间：
1993-06-01
影响因子：
3.2
作者：
HAMRE, KM;WEST, JR
通讯作者：
WEST, JR
DOI：
10.1097/00000374-199706000-00028
发表时间：
1997-06-01
影响因子：
3.2
作者：
Goodlett, CR;Eilers, AT
通讯作者：
Eilers, AT
影响因子：
5.3
作者：
Fagel, Devon M.;Ganat, Yosif;Vaccarino, Flora M.
通讯作者：
Vaccarino, Flora M.
影响因子：
2.9
作者：
Green, JT;Tran, T;Goodlett, CR
通讯作者：
Goodlett, CR