Onset of chromatin fragmentation in chloroma cell apoptosis is highly sensitive to UV and begins at non-B DNA conformation.
Onset of chromatin fragmentation in chloroma cell apoptosis is highly sensitive to UV and begins at non-B DNA conformation.
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绿色瘤细胞凋亡中染色质断裂的发生对紫外线高度敏感，并从非 B DNA 构象开始。
DOI：
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发表时间：
1993
影响因子：
2.6
通讯作者：
T. Rytäomaa
中科院分区：
文献类型：
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作者：
M. Luokkamäki;K. Servomaa;T. Rytäomaa
UV irradiation induces programmed death, or apoptosis, in rat chloroleukaemia cells, a process characterized by typical cell morphology, fragmentation of chromatin into units of single and multiple nucleosomes, and transcriptional activation of LINE. Our study shows that this process is initiated by very low UV doses: exposure to one minimal erythema dose (MED, about 200 J/m2), at defined wavelengths ranging from 270 to 330 nm, is sufficient. By sequencing 100 randomly selected blunt-end chromatin fragments (single and multiple nucleosomes) we observed that at an early stage of apoptosis the fragmented DNA contains a four-fold excess of both long and short interspersed nuclear retroelements (LINEs and SINEs). A distinct sequence finding was also the observation that fragmented DNA is very rich in microsatellites, (CA)n dinucleotides in particular, and in long stretches of homopurine/homopyrimidine domains. The present results thus indicate that chromatin fragmentation in UV-induced apoptosis begins at non-random locations involving non-B DNA conformation, and that the onset of the suicide process in chloroleukaemia cells is surprisingly sensitive to UV exposure.
DOI：
10.1073/pnas.81.3.713
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
MILNER, RJ;BLOOM, FE;SUTCLIFFE, JG
通讯作者：
SUTCLIFFE, JG
小鼠中的 L1Md 长散布重复序列家族：几乎所有示例都在一端被截断。
影响因子：
14.9
作者：
Voliva,CF;Jahn,CL;Comer,MB;Hutchison3rd,CA;Edgell,MH
通讯作者：
Edgell,MH