Transcriptome Profiling Reveals Enhanced Mitochondrial Activity as a Cold Adaptive Strategy to Hypothermia in Zebrafish Muscle.
Transcriptome Profiling Reveals Enhanced Mitochondrial Activity as a Cold Adaptive Strategy to Hypothermia in Zebrafish Muscle.
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DOI：
10.3390/cells12101366
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发表时间：
2023-05-11
期刊：
影响因子：
6
通讯作者：
Hardiman, Gary
中科院分区：
文献类型：
--
作者：
Cahill, Thomas;Chan, Sherine;Overton, Ian M.;Hardiman, Gary
利用人造休眠状态进行星际旅行曾经看起来很牵强。然而，越来越多的证据表明，太空旅行的主要危险，即暴露于辐射和微重力环境，也会给火炬带来保护性益处。为了确定诱导的torpor-like状态的辐射保护作用，我们利用了斑马鱼（斑马鱼）在降低体温时的体温外热性质，以复制自然休眠期间所见的低温状态。我们还给予褪黑激素作为镇静剂，以减少体力活动。然后将斑马鱼暴露于低剂量辐射（0.3戈伊），以模拟长期空间飞行任务中的辐射暴露。转录组学分析发现，辐射暴露导致炎症和免疫特征的上调以及由STAT 3和MYOD 1转录因子驱动的分化和再生表型。此外，DNA修复过程在照射后两天的肌肉中下调。低温的影响导致线粒体翻译的增加，包括参与氧化磷酸化的基因和细胞外基质和发育基因的下调。在辐射暴露后，在休眠+辐射组中观察到内质网应激基因增加，免疫相关和ECM基因下调。暴露于辐射的低温斑马鱼也导致ECM和发育基因的下调，然而，与仅辐射组中观察到的相反，免疫/炎症相关通路下调。一个跨物种的比较进行冬眠的熊（棕熊）的肌肉，以确定共同的耐冷机制。共享的反应显示蛋白质翻译和氨基酸代谢的上调，以及糖酵解、ECM和发育基因共享下调的缺氧反应。
The utilisation of synthetic torpor for interplanetary travel once seemed farfetched. However, mounting evidence points to torpor-induced protective benefits from the main hazards of space travel, namely, exposure to radiation and microgravity. To determine the radio-protective effects of an induced torpor-like state we exploited the ectothermic nature of the Danio rerio (zebrafish) in reducing their body temperatures to replicate the hypothermic states seen during natural torpor. We also administered melatonin as a sedative to reduce physical activity. Zebrafish were then exposed to low-dose radiation (0.3 Gy) to simulate radiation exposure on long-term space missions. Transcriptomic analysis found that radiation exposure led to an upregulation of inflammatory and immune signatures and a differentiation and regeneration phenotype driven by STAT3 and MYOD1 transcription factors. In addition, DNA repair processes were downregulated in the muscle two days’ post-irradiation. The effects of hypothermia led to an increase in mitochondrial translation including genes involved in oxidative phosphorylation and a downregulation of extracellular matrix and developmental genes. Upon radiation exposure, increases in endoplasmic reticulum stress genes were observed in a torpor+radiation group with downregulation of immune-related and ECM genes. Exposing hypothermic zebrafish to radiation also resulted in a downregulation of ECM and developmental genes however, immune/inflammatory related pathways were downregulated in contrast to that observed in the radiation only group. A cross-species comparison was performed with the muscle of hibernating Ursus arctos horribilis (brown bear) to define shared mechanisms of cold tolerance. Shared responses show an upregulation of protein translation and metabolism of amino acids, as well as a hypoxia response with the shared downregulation of glycolysis, ECM, and developmental genes.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
5.6
作者：
Cahill T;Cope H;Bass JJ;Overbey EG;Gilbert R;da Silveira WA;Paul AM;Mishra T;Herranz R;Reinsch SS;Costes SV;Hardiman G;Szewczyk NJ;Tahimic CGT
通讯作者：
Tahimic CGT
影响因子：
5.3
作者：
Dai, Yi;Liang, Shengran;Banerjee, Santasree
通讯作者：
Banerjee, Santasree
影响因子：
3.4
作者：
Bandstra, Eric R.;Thompson, Raymond W.;Bateman, Ted A.
通讯作者：
Bateman, Ted A.
同时存在的BMP信号传导维持和TGF-β信号抑制是冬眠熊对肌肉萎缩的自然抗性的标志。
影响因子：
6
作者：
Cussonneau L;Boyer C;Brun C;Deval C;Loizon E;Meugnier E;Gueret E;Dubois E;Taillandier D;Polge C;Béchet D;Gauquelin-Koch G;Evans AL;Arnemo JM;Swenson JE;Blanc S;Simon C;Lefai E;Bertile F;Combaret L
通讯作者：
Combaret L