A microarray analysis of sexual dimorphism of adipose tissues in high-fat-diet-induced obese mice.
A microarray analysis of sexual dimorphism of adipose tissues in high-fat-diet-induced obese mice.
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DOI：
10.1038/ijo.2010.12
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发表时间：
2010-06
影响因子：
4.9
通讯作者：
Clegg, D. J.
中科院分区：
文献类型：
--
作者：
Grove, K. L.;Fried, S. K.;Greenberg, A. S.;Xiao, X. Q.;Clegg, D. J.
性二态性存在于身体脂肪分布;女性存款相对较多的脂肪在皮下/腹股沟仓库，而男性存款更多的脂肪在腹腔内/性腺仓库。我们的目的是系统地记录贮库和性别相关的差异积累的脂肪组织和基因表达，比较差异表达的基因在饮食诱导的肥胖小鼠与小鼠维持在普通饲料饮食。我们使用微阵列的方法来确定是否有性别的基因表达在年龄匹配的男性，女性或卵巢切除的女性（OVX）C57/BL 6小鼠维持高脂肪（HF）饮食。然后，我们比较了性别之间的验证基因的表达在食物饮食。在暴露于高脂肪饮食12周后，雌性的体重增加少于雄性。微阵列分析表明，在女性的腹内/性腺脂肪组织中，1642个基因在贮库之间至少有两倍的差异，而与男性相比，皮下/腹股沟脂肪组织中有706个基因不同。只有138个基因在两性和脂肪组织库中受到共同调控。与女性相比，男性中的炎症基因（精氨酸-细胞因子受体相互作用和急性期蛋白合成）上调，并且在OVX后存在部分逆转，其中OVX脂肪组织基因表达更“男性样”。在维持食物的小鼠中未观察到这种模式。雄性性腺白色脂肪组织（GWAT）的组织学显示出比雌性更多的冠状结构，表明炎症和脂肪组织重塑。此外，与胰岛素信号和脂质合成相关的基因在女性中高于男性，无论饮食暴露如何。这些数据表明，男性和女性的脂肪组织在性别之间存在差异，无论饮食如何。此外，HF饮食暴露在男性中激发了比女性更大的炎症反应。该数据集强调了分析脂肪组织分布和功能的贮库、性别和类固醇依赖性调节的重要性。
A sexual dimorphism exists in body fat distribution; females deposit relatively more fat in subcutaneous/inguinal depots whereas males deposit more fat in the intra-abdominal/gonadal depot. Our objective was to systematically document depot- and sex-related differences in the accumulation of adipose tissue and gene expression, comparing differentially expressed genes in diet-induced obese mice with mice maintained on a chow diet. We used a microarray approach to determine whether there are sexual dimorphisms in gene expression in age-matched male, female or ovariectomized female (OVX) C57/BL6 mice maintained on a high-fat (HF) diet. We then compared expression of validated genes between the sexes on a chow diet. After exposure to a high fat diet for 12 weeks, females gained less weight than males. The microarray analyses indicate in intra-abdominal/gonadal adipose tissue in females 1642 genes differ by at least twofold between the depots, whereas 706 genes differ in subcutaneous/inguinal adipose tissue when compared with males. Only 138 genes are commonly regulated in both sexes and adipose tissue depots. Inflammatory genes (cytokine–cytokine receptor interactions and acute-phase protein synthesis) are upregulated in males when compared with females, and there is a partial reversal after OVX, where OVX adipose tissue gene expression is more ′male-like′. This pattern is not observed in mice maintained on chow. Histology of male gonadal white adipose tissue (GWAT) shows more crown-like structures than females, indicative of inflammation and adipose tissue remodeling. In addition, genes related to insulin signaling and lipid synthesis are higher in females than males, regardless of dietary exposure. These data suggest that male and female adipose tissue differ between the sexes regardless of diet. Moreover, HF diet exposure elicits a much greater inflammatory response in males when compared with females. This data set underscores the importance of analyzing depot-, sex- and steroid-dependent regulation of adipose tissue distribution and function.
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DOI：
10.1016/0304-4165(72)90248-6
发表时间：
1972-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
GEISLER, RW;HANSEN, RJ
通讯作者：
HANSEN, RJ
影响因子：
1.7
作者：
Doniger, S;Hofmann, T;Yeh, J
通讯作者：
Yeh, J
影响因子：
7.7
作者：
Hevener, A;Reichart, D;Olefsky, J
通讯作者：
Olefsky, J
影响因子：
4.8
作者：
D'Eon, TM;Souza, SC;Greenberg, AS
通讯作者：
Greenberg, AS
影响因子：
9.8
作者：
Boivin, Ariane;Brochu, Gaetan;Tchernof, Andre
通讯作者：
Tchernof, Andre