Distinct Chemotaxis Protein Paralogs Assemble into Chemoreceptor Signaling Arrays To Coordinate Signaling Output
Distinct Chemotaxis Protein Paralogs Assemble into Chemoreceptor Signaling Arrays To Coordinate Signaling Output
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不同的趋化蛋白旁系同源物组装成化学感受器信号阵列以协调信号输出
DOI：
10.1128/mbio.01757-19
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发表时间：
2019
期刊：
影响因子：
6.4
通讯作者：
Peter Greenberg, E.
中科院分区：
文献类型：
--
作者：
O’Neal, Lindsey;Gullett, Jessica M.;Aksenova, Anastasia;Hubler, Adam;Briegel, Ariane;Ortega, Davi;Kjær, Andreas;Jensen, Grant;Alexandre, Gladys;Peter Greenberg, E.
大多数趋化运动细菌具有多种趋化性信号系统，其功能还不清楚。趋化性信号传导由组装成大阵列的化学受体以及趋化性偶联蛋白（CheW）和组氨酸激酶蛋白（CheA）启动，所述趋化性偶联蛋白和组氨酸激酶蛋白与受体的细胞质尖端形成基板。这些细胞极定位阵列介导趋化反应期间的传感、信号传导和信号放大。具有不同胞质结构域长度的膜结合化学感受器分离成不同的阵列。在这里，我们表明，一种细菌，巴西固氮菌，它利用两个趋化性信号系统控制不同的运动参数，协调其趋化反应，通过生产两个单独的膜结合的化学感受器阵列混合旁系同源物内的趋化性基板。不同长度类的化学感受器的极性定位保持在具有来自任一趋化系统的基板信号蛋白的菌株中，但当两个系统都被删除时丢失。来自每个趋化性信号系统（Che 1和Che 4）的趋化性蛋白（Che A和Che W）可以彼此物理相互作用，并且A中存在来自两个类别的化学受体。brasilensec可以与Che 1和Che 4蛋白相互作用。从不同的趋化性途径到基板的旁系同源物的组装提供了一个简单的机制，协调信号从不同的途径，我们预测是不是唯一的这个系统的倾向，趋化性系统的水平genetransfer.IMPORTANCEThe组装的趋化性受体和信号蛋白成极性阵列是普遍的运动趋化性细菌。比较基因组分析表明，大多数能动细菌具有多种趋化性信号系统，实验证据表明，从不同的趋化性系统的信号是集成的。在这里，我们确定一个这样的机制。我们发现，从两个趋化系统的旁系同源物组装在一起的化学感受器阵列，形成基板组成的蛋白质从两个趋化系统。这些混合阵列提供了一个直接的机制，信号整合和协调反应输出不同的趋化系统。考虑到大多数趋化性细菌编码多种趋化性系统，并且这些系统倾向于横向转移，这种机制可能是常见的，以确保趋化性信号整合发生。
Most chemotactic motile bacteria possess multiple chemotaxis signaling systems, the functions of which are not well characterized. Chemotaxis signaling is initiated by chemoreceptors that assemble as large arrays, together with chemotaxis coupling proteins (CheW) and histidine kinase proteins (CheA), which form a baseplate with the cytoplasmic tips of receptors. These cell pole-localized arrays mediate sensing, signaling, and signal amplification during chemotaxis responses. Membrane-bound chemoreceptors with different cytoplasmic domain lengths segregate into distinct arrays. Here, we show that a bacterium, Azospirillum brasilense, which utilizes two chemotaxis signaling systems controlling distinct motility parameters, coordinates its chemotactic responses through the production of two separate membrane-bound chemoreceptor arrays by mixing paralogs within chemotaxis baseplates. The polar localization of chemoreceptors of different length classes is maintained in strains that had baseplate signaling proteins from either chemotaxis system but was lost when both systems were deleted. Chemotaxis proteins (CheA and CheW) from each of the chemotaxis signaling systems (Che1 and Che4) could physically interact with one another, and chemoreceptors from both classes present inA. brasilensecould interact with Che1 and Che4 proteins. The assembly of paralogs from distinct chemotaxis pathways into baseplates provides a straightforward mechanism for coordinating signaling from distinct pathways, which we predict is not unique to this system given the propensity of chemotaxis systems for horizontal gene transfer.IMPORTANCEThe assembly of chemotaxis receptors and signaling proteins into polar arrays is universal in motile chemotactic bacteria. Comparative genome analyses indicate that most motile bacteria possess multiple chemotaxis signaling systems, and experimental evidence suggests that signaling from distinct chemotaxis systems is integrated. Here, we identify one such mechanism. We show that paralogs from two chemotaxis systems assemble together into chemoreceptor arrays, forming baseplates comprised of proteins from both chemotaxis systems. These mixed arrays provide a straightforward mechanism for signal integration and coordinated response output from distinct chemotaxis systems. Given that most chemotactic bacteria encode multiple chemotaxis systems and the propensity for these systems to be laterally transferred, this mechanism may be common to ensure chemotaxis signal integration occurs.
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重新利用大分子机器：F7 化学感应系统的架构和演化
DOI：
10.1101/653600
发表时间：
2019
期刊：
bioRxiv
影响因子：
--
作者：
Davi R. Ortega;P. Subramanian;P. Mann;A. Kjær;Songye Chen;Kylie J. Watts;Sahand Pirbadian;David A. Collins;R. Kooger;M. Kalyuzhnaya;S. Ringgaard;A. Briegel;G. Jensen
通讯作者：
G. Jensen
影响因子：
3.3
作者：
Ouellette, Scot P.;Gauliard, Emilie;Ladant, Daniel
通讯作者：
Ladant, Daniel
影响因子：
2.9
作者：
Natale, Andrew M.;Duplantis, Jane L.;Piasta, Kene N.;Falke, Joseph J.
通讯作者：
Falke, Joseph J.
影响因子：
4.4
作者：
Hallez, Regis;Letesson, Jean-Jacques;De Bolle, Xavier
通讯作者：
De Bolle, Xavier
影响因子：
10.7
作者：
Katoh K;Standley DM
通讯作者：
Standley DM