Reduced bacterial adhesion to fibrinogen-coated substrates via nitric oxide release.
Reduced bacterial adhesion to fibrinogen-coated substrates via nitric oxide release.
复制标题
DOI：
10.1016/j.biomaterials.2008.07.005
复制
发表时间：
2008-10
期刊：
影响因子：
14
通讯作者：
Schoenfisch, Mark H.
中科院分区：
文献类型：
--
作者：
Charville, Gregory W.;Hetrick, Evan M.;Geer, Carri B.;Schoenfisch, Mark H.
一氧化氮(NO)释放的干凝胶能够减少纤维蛋白原介导的金黄色葡萄球菌、表皮葡萄球菌和大肠杆菌之间的黏附。在所测试的每个物种中，NO表面通量与细菌粘附性之间呈负相关。对于金黄色葡萄球菌和大肠埃希菌，黏附力的降低与NO释放量在0~30pmocm cm−2 S−1之间呈正相关。对表皮葡萄球菌也有类似的依赖关系。在NO释放量为30pmolcm−2 S−1时，金黄色葡萄球菌、表皮葡萄球菌和大肠杆菌的表面覆盖率分别比未释放NO的对照减少了96%、48%和88%。因此，聚合物NO释放被证明是一种在体外显著降低纤维蛋白原介导的革兰氏阳性和革兰氏阴性细菌黏附的有效方法，从而说明了在存在预吸附蛋白的情况下，活性NO释放作为一种抑制细菌黏附的策略的优势。
The ability of nitric oxide (NO)-releasing xerogels to reduce fibrinogen-mediated adhesion of Staphylococcus aureus, Staphylococcus epidermidis, and Escherichia coli is described. A negative correlation was observed between NO surface flux and bacterial adhesion for each species tested. For S. aureus and E. coli, reduced adhesion correlated directly with NO flux from 0 to 30 pmol cm−2 s−1. A similar dependence for S. epidermidis was evident from 18 to 30 pmol cm−2 s−1. At a NO flux of 30 pmol cm−2 s−1, surface coverage of S. aureus, S. epidermidis, and E. coli was reduced by 96, 48, and 88%, respectively, compared to non-NO-releasing controls. Polymeric NO release was thus demonstrated to be an effective approach for significantly reducing fibrinogen-mediated adhesion of both gram-positive and gram-negative bacteria in vitro, thereby illustrating the advantage of active NO release as a strategy for inhibiting bacterial adhesion in the presence of pre-adsorbed protein.
登录
查看更多内容
影响因子：
14
作者：
Kumar, R;Münstedt, H
通讯作者：
Münstedt, H
影响因子：
3.1
作者：
Darling, KEA;Evans, TJ
通讯作者：
Evans, TJ
影响因子：
3.2
作者：
Katsaros, D;Manolopoulos, Y
通讯作者：
Manolopoulos, Y
影响因子：
6.2
作者：
Nablo, BJ;Schoenfisch, MH
通讯作者：
Schoenfisch, MH
影响因子：
4.9
作者：
Nablo, BJ;Schoenfisch, MH
通讯作者：
Schoenfisch, MH