Epithelial–mesenchymal transformation and apoptosis in rat urethra development
Epithelial–mesenchymal transformation and apoptosis in rat urethra development
复制标题
大鼠尿道发育中的上皮-间质转化和细胞凋亡
DOI：
10.1038/pr.2017.185
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发表时间：
2017-09
影响因子：
3.6
通讯作者：
魏光辉
中科院分区：
文献类型：
--
作者：
周玥;刘星;黄方圆;刘洋;曹希宁;沈炼桔;龙春兰;何大维;林涛;魏光辉
背景。目的探讨大鼠尿道胚胎发育过程中尿道缝形成的机制。在胚胎日（ED） 15、16、18和19日处死时间交配的Sprague-Dawley大鼠，取雄性幼鼠生殖器结节。扫描电镜下观察其外部形态。采用苏木精-伊红染色、免疫组化、透射电镜、双免疫荧光等方法观察尿道缝形态的动态变化。从ED 16开始观察到双侧尿道肿胀，随后中线尿道板融合形成尿道缝。在尿道缝的几个细胞中观察到上皮和间充质标记物的共表达；少数细胞同时表达上皮和凋亡标记。透射电镜下观察上皮细胞间质转化及上皮细胞凋亡。尿道的形成是通过小管形成发生的，小管形成始于尿道近端，并向尿道远端发展。这是首次证实胎鼠尿道缝细胞上皮间质转化和上皮细胞凋亡的研究。这些发现为尿道胚胎发育的机制提供了新的见解。
Background.To examine the mechanism of urethral seam formation during embryonal development of rat urethra...Methods.Time-mated Sprague–Dawley rats were killed and the genital tubercles of male pups harvested on embryonic day (ED) 15, 16, 18, and 19. External morphology was observed under scanning electron microscope. Serial transverse sections were prepared to examine dynamic changes in the urethral seam morphology with hematoxylin–eosin staining, immunohistochemistry, transmission electron microscopy, and double immunofluorescence...Results.Bilateral outgrowth of urethral swelling followed by urethral plate fusion in the midline to form urethral seam was observed from ED 16 onwards. Coexpression of epithelial and mesenchymal markers was observed in several cells at the urethral seam; a few cells with coexpression of epithelial and apoptotic markers were also observed. Mesenchymal transformation of epithelial cells and apoptotic epithelial cells was observed under transmission electron microscope...Conclusion.Urethral formation occurs by tubulogenesis, which initiates proximally and progresses distally. This is the first study to demonstrate epithelial–mesenchymal transformation and epithelial cell apoptosis in the urethral seam cells of fetal rats. These findings provide new insights into the mechanisms involved in embryonal development of the urethra.
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影响因子：
--
作者：
F. Wood-Jones
通讯作者：
F. Wood-Jones
影响因子：
3.6
作者：
Baskin, LS;Erol, A;Cunha, GR
通讯作者：
Cunha, GR
影响因子：
2.4
作者：
BY T. W. Glenister;Charing Cross;T. Glenister
通讯作者：
BY T. W. Glenister;Charing Cross;T. Glenister
影响因子：
2.4
作者：
Romia Hassoun;P. Schwartz;D. Rath;C. Viebahn;J. Männer
通讯作者：
Romia Hassoun;P. Schwartz;D. Rath;C. Viebahn;J. Männer
影响因子：
10
作者：
GLUCKSMANN, A
通讯作者：
GLUCKSMANN, A