The expression of glutamate aspartate transporter (GLAST) within the human cochlea and its distribution in various patient populations.
The expression of glutamate aspartate transporter (GLAST) within the human cochlea and its distribution in various patient populations.
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DOI：
10.1016/j.brainres.2013.06.040
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发表时间：
2013-09-05
期刊：
影响因子：
2.9
通讯作者：
Ishiyama A
中科院分区：
文献类型：
--
作者：
Ahmed S;Vorasubin N;Lopez IA;Hosokawa S;Ishiyama G;Ishiyama A
Glutamate plays an important role in the central nervous system as an excitatory neurotransmitter. However, its abundance can lead to excitotoxicity which necessitates the proper function of active glutamate transporters. The glutamate-aspartate transporter (GLAST) has been shown to exist and function within non-human cochlear specimens regulating the inner ear glutamate concentration. In this study, we examined micro-dissected human cochleas from formalin-fixed celloidin-embedded temporal bone specimens of three different types of patients (Meniere's disease, normal controls, and other otopathologic conditions) and examined the differential expression of GLAST in the spiral ligament of the basal, middle, and apical turns of the cochlea. Immunohistochemical staining was performed with polyclonal antibodies against GLAST and image analysis was carried out with the Image J analysis software. In contrast to other studies with non-human specimens, GLAST was expressed in the spiral ligament fibrocytes but was not detected in the satellite cells of the spiral ganglia or supporting cells of the organ of Corti in the human cochlea. Our data also showed that GLAST expression significantly differs in the basal and apical turns of the cochlea. Lastly, post-hoc analysis showed a difference in the GLAST immunoreactive area of patients with Meniere's disease when compared to that of patients with other otopathologic conditions—such as presbycusis or ototoxicity. These results may potentially lead to further understanding of different disease states that affect hearing.
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影响因子：
2.5
作者：
Rangan, Gopala K.;Tesch, Greg H.
通讯作者：
Tesch, Greg H.
影响因子：
3.6
作者：
Calzada, Audrey P.;Lopez, Ivan A.;Ishiyama, Gail
通讯作者：
Ishiyama, Gail
DOI：
10.1002/ar.22622
发表时间：
2013-02-01
影响因子：
2
作者：
Balaker, Ashley E.;Ishiyama, Paul;Ishiyama, Akira
通讯作者：
Ishiyama, Akira
影响因子：
3.4
作者：
Furness, DN;Lehre, KP
通讯作者：
Lehre, KP
影响因子：
1.4
作者：
JOHNSSON, LG;HAWKINS, JE
通讯作者：
HAWKINS, JE