Salt Stress Promotes Abscisic Acid Accumulation to Affect Cell Proliferation and Expansion of Primary Roots in Rice.
Salt Stress Promotes Abscisic Acid Accumulation to Affect Cell Proliferation and Expansion of Primary Roots in Rice.
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盐胁迫促进水稻脱落酸积累影响细胞增殖和初生根扩张
DOI：
10.3390/ijms221910892
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发表时间：
2021-10-08
影响因子：
5.6
通讯作者：
Qin H
中科院分区：
文献类型：
--
作者：
Huang Y;Zhou J;Li Y;Quan R;Wang J;Huang R;Qin H
初生根是水稻根系的基本组成部分，在水稻早期幼苗生长中起着关键作用。它的生长很容易受到环境因素的影响，例如作为盐胁迫。脱落酸（阿坝）在根的发育中起着重要的作用，但ABA调控根生长响应盐胁迫的分子机制仍然知之甚少。在这项研究中，我们报告说，盐胁迫抑制主根伸长，促进主根肿胀。此外，盐胁迫诱导ABA响应基因的表达和阿坝积累的初生根，揭示阿坝在盐调节根生长中起着重要作用。组成型表达OsSAPK 10或OsABIL 2的OsSAPK 10-OE和OsABIL 2-OE的转基因系，具有增强或减弱的阿坝信号传导，相应地显示对盐的敏感性增加和降低。显微镜分析表明，盐和阿坝抑制细胞增殖，促进根尖分生组织中的细胞扩展。转录组分析表明，阿坝诱导EXPANSIN基因的表达。进一步的研究表明，阿坝发挥这些作用主要是通过阿坝信号。因此，我们的研究结果加深了我们的理解阿坝在盐胁迫下控制初生根生长的作用，这方面的知识可以用于育种家培育适合盐碱地的水稻品种。
The primary root is the basic component of the root system and plays a key role in early seedling growth in rice. Its growth is easily affected by environmental cues, such as salt stress. Abscisic acid (ABA) plays an essential role in root development, but the molecular mechanism underlying ABA-regulated root growth in response to salt stress remains poorly understood. In this study, we report that salt stress inhibits primary root elongation and promotes primary root swelling. Moreover, salt stress induces the expression of ABA-responsive genes and ABA accumulation in the primary root, revealing that ABA plays an essential role in salt-modulated root growth. Transgenic lines of OsSAPK10-OE and OsABIL2-OE, which constitutively express OsSAPK10 or OsABIL2, with enhanced or attenuated ABA signaling, show increased and decreased sensitivity to salt, correspondingly. Microscopic analysis indicates that salt and ABA inhibits cell proliferation and promotes cell expansion in the root apical meristem. Transcriptome analysis showed that ABA induces the expression of EXPANSIN genes. Further investigations indicate that ABA exerts these effects largely through ABA signaling. Thus, our findings deepen our understanding of the role of ABA in controlling primary root growth in response to salt stress, and this knowledge can be used by breeders to cultivate rice varieties suitable for saline–alkali land.
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影响因子：
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作者：
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DOI：
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发表时间：
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期刊：
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影响因子：
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