Overexpression of OsEXPA8, a root-specific gene, improves rice growth and root system architecture by facilitating cell extension.
Overexpression of OsEXPA8, a root-specific gene, improves rice growth and root system architecture by facilitating cell extension.
复制标题
根特异性基因 OsEXPA8 的过度表达可促进细胞延伸，从而改善水稻生长和根系结构
DOI：
10.1371/journal.pone.0075997
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Huang J
中科院分区：
文献类型：
--
作者：
Ma N;Wang Y;Qiu S;Kang Z;Che S;Wang G;Huang J
扩展蛋白是植物细胞壁的一种特殊蛋白质，参与植物细胞壁的修饰。本研究通过转基因水稻研究了根特异性α-expansin基因OsEXPA 8在水稻生长发育中可能的生物学作用。OsEXPA 8在水稻植株中的过表达产生了改善的根系结构（更长的初生根、更多的侧根和根毛）、增加的植株高度、增加的叶片数量和增大的叶片大小的多效性表型。进一步的研究表明，OsEXPA 8诱导的水稻叶片和根系维管束细胞平均长度增加，悬浮培养细胞生长量增加，揭示了OsEXPA 8促进水稻植株生长的细胞学基础。膨胀素被认为是细胞扩大和壁松弛所需的关键因素。原子力显微镜（AFM）技术揭示，在不同生长阶段，与野生型对应物相比，35 S：：OsEXPA 8转基因悬浮培养细胞的平均壁刚度值降低超过6倍。此外，观察到悬浮细胞壁聚合物组成的显著变化，傅里叶变换红外（FTIR）光谱显示OsEXPA 8过表达细胞壁组成中多糖/木质素含量的比率相对增加。这些结果支持扩展蛋白在细胞扩增和植物生长中的作用。
Expansins are unique plant cell wall proteins that are involved in cell wall modifications underlying many plant developmental processes. In this work, we investigated the possible biological role of the root-specific α-expansin gene OsEXPA8 in rice growth and development by generating transgenic plants. Overexpression of OsEXPA8 in rice plants yielded pleiotropic phenotypes of improved root system architecture (longer primary roots, more lateral roots and root hairs), increased plant height, enhanced leaf number and enlarged leaf size. Further study indicated that the average cell length in both leaf and root vascular bundles was enhanced, and the cell growth in suspension cultures was increased, which revealed the cellular basis for OsEXPA8-mediated rice plant growth acceleration. Expansins are thought to be a key factor required for cell enlargement and wall loosening. Atomic force microscopy (AFM) technology revealed that average wall stiffness values for 35S::OsEXPA8 transgenic suspension-cultured cells decreased over six-fold compared to wild-type counterparts during different growth phases. Moreover, a prominent change in the wall polymer composition of suspension cells was observed, and Fourier-transform infrared (FTIR) spectra revealed a relative increase in the ratios of the polysaccharide/lignin content in cell wall compositions of OsEXPA8 overexpressors. These results support a role for expansins in cell expansion and plant growth.
登录
查看更多内容
DOI：
10.1073/pnas.94.12.6559
发表时间：
1997-06-10
影响因子：
11.1
作者：
Cosgrove, DJ;Bedinger, P;Durachko, DM
通讯作者：
Durachko, DM
影响因子：
41.2
作者：
Keckes, J;Burgert, I;Fratzl, P
通讯作者：
Fratzl, P
影响因子：
7.8
作者：
Leppard, G G;Colvin, J R;Rose, D;Martin, S M
通讯作者：
Martin, S M
影响因子：
11.6
作者：
Cho, HT;Cosgrove, DJ
通讯作者：
Cosgrove, DJ
影响因子：
11.6
作者：
Choi, DS;Lee, Y;Kende, H
通讯作者：
Kende, H