Differentially expressed proteins and associated histological and disease progression changes in cotyledon tissue of a resistant and susceptible genotype of brassica napus infected with Sclerotinia sclerotiorum.
Differentially expressed proteins and associated histological and disease progression changes in cotyledon tissue of a resistant and susceptible genotype of brassica napus infected with Sclerotinia sclerotiorum.
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DOI：
10.1371/journal.pone.0065205
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Barbetti MJ
中科院分区：
文献类型：
--
作者：
Garg H;Li H;Sivasithamparam K;Barbetti MJ
由核盘菌引起的菌核腐病是油菜最严重的病害之一。为了了解甘蓝型油菜对核盘菌的抗性机制，对两种甘蓝型油菜基因型（抗性 cv. Charlton，易感 cv. RQ001-02M2）进行了比较疾病进展、组织学和蛋白质组学研究。接种后 72 和 96 小时 (hpi)，抗性 Charlton 子叶上的病斑尺寸显着较小 (P≤0.001)。解剖学研究显示，仅在抗性查尔顿上真菌生长受阻（24 hpi 及以后）和菌丝崩解。蛋白质谱的时间变化（12、24、48和72 hpi）显示某些酶仅在抗性Charlton中上调，例如与主要代谢途径、抗氧化防御、乙烯生物合成、发病机制相关蛋白质、蛋白质合成和蛋白质折叠相关的酶，在介导针对核盘菌的防御反应中发挥作用。类似地，真核翻译起始因子 5A 酶在易感 RQ001-02M2 中丰度增加，而在抗性 Charlton 中水平降低，在增加对该病原体的易感性方面发挥作用。这是第一次在蛋白质组学水平上证明这些酶的表达与介导欧洲油菜抗性品种子叶组织中针对核盘菌的防御反应有关。这项研究不仅为甘蓝型油菜对核盘菌的抗性机制提供了重要的新见解，而且为新的甘蓝型油菜品种的新型工程开辟了道路，这些品种过度表达这些关键酶，作为增强抗性和更好地管理这种破坏性病原体的策略。
Sclerotinia rot caused by Sclerotinia sclerotiorum is one of the most serious diseases of oilseed rape. To understand the resistance mechanisms in the Brassica napus to S. sclerotiorum, comparative disease progression, histological and proteomic studies were conducted of two B. napus genotypes (resistant cv. Charlton, susceptible cv. RQ001-02M2). At 72 and 96 h post inoculation (hpi), lesion size on cotyledons was significantly (P≤0.001) smaller in the resistant Charlton. Anatomical investigations revealed impeded fungal growth (at 24 hpi and onwards) and hyphal disintegration only on resistant Charlton. Temporal changes (12, 24, 48 and 72 hpi) in protein profile showed certain enzymes up-regulated only in resistant Charlton, such as those related to primary metabolic pathways, antioxidant defence, ethylene biosynthesis, pathogenesis related proteins, protein synthesis and protein folding, play a role in mediating defence responses against S. sclerotiorum. Similarly a eukaryotic translation initiation factor 5A enzyme with increased abundance in susceptible RQ001-02M2 and decreased levels in resistant Charlton has a role in increased susceptibility to this pathogen. This is the first time that the expression of these enzymes has been shown to be associated with mediating the defence response against S. sclerotinia in cotyledon tissue of a resistant cultivar of B. napus at a proteomics level. This study not only provides important new insights into the resistance mechanisms within B. napus against S. sclerotiorum, but opens the way for novel engineering of new B. napus varieties that over-express these key enzymes as a strategy to enhance resistance and better manage this devastating pathogen.
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影响因子：
4.3
作者：
EDWARDS, R;BLOUNT, JW;DIXON, RA
通讯作者：
DIXON, RA
影响因子：
4.8
作者：
Gazaryan, IG;Krasnikov, BF;Brown, AM
通讯作者：
Brown, AM
影响因子：
5.8
作者：
Ge, Xin Tian;Li, Yu Pin;Barbetti, Martin J.
通讯作者：
Barbetti, Martin J.
影响因子：
11.6
作者：
Cessna, SG;Sears, VE;Low, PS
通讯作者：
Low, PS
影响因子：
2.7
作者：
GODOY, G;STEADMAN, JR;DAM, R
通讯作者：
DAM, R