Chronic and acute alcohol administration induced neurochemical changes in the brain: comparison of distinct zebrafish populations.
Chronic and acute alcohol administration induced neurochemical changes in the brain: comparison of distinct zebrafish populations.
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DOI：
10.1007/s00726-013-1658-y
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发表时间：
2014-04
期刊：
影响因子：
3.5
通讯作者：
Gerlai, Robert
中科院分区：
文献类型：
--
作者：
Chatterjee, Diptendu;Shams, Soaleha;Gerlai, Robert
斑马鱼越来越多地被用于分析乙醇（酒精）对大脑功能和行为的影响。我们已经证明了酒精对斑马鱼行为的显著影响，并开始分析多巴胺能和血清素能反应的变化。在这里，我们使用2×3（慢性x急性）随机分析了两个斑马鱼种群AB和SF的受试者酒精暴露范式之间酒精对选定神经化学物质水平的影响。每条鱼首先接受特定的慢性处理（0或0.5 vol/vol%酒精），随后接受急性暴露（0、0.5或1.0%酒精）。我们报告了用高效液相色谱法定量测定全脑提取物中多巴胺、多巴胺、血清素、5HIAA、谷氨酸、GABA、天冬氨酸、甘氨酸和牛磺酸水平的变化。我们还分析了单胺氧化酶（MAO）和酪氨酸羟化酶（TH）的酶活性。结果表明，与SF相比，AB在神经化学水平上对急性酒精暴露和急性酒精戒断都更敏感，这一发现与先前的行为观察有很好的相关性，并且表明所观察到的酒精效应与基因有关。我们讨论了当前结果与先前行为发现之间的相关性，并强调了斑马鱼品系表征对未来行为遗传和精神药理学研究的重要性。
The zebrafish is increasingly utilized in the analysis of the effects of ethanol (alcohol) on brain function and behaviour. We have shown significant population dependent alcohol induced changes in zebrafish behaviour and have started to analyze alterations in dopaminergic and serotoninergic responses. Here, we analyze the effects of alcohol on levels of selected neurochemicals using a 2×3 (chronic x acute) between subject alcohol exposure paradigm randomized for two zebrafish populations, AB and SF. Each fish first received the particular chronic treatment (0 or 0.5 vol/vol% alcohol) and subsequently the acute exposure (0, 0.5 or 1.0% alcohol). We report changes in levels of dopamine, DOPAC, serotonin, 5HIAA, glutamate, GABA, aspartate, glycine and taurine as quantified from whole brain extracts using HPLC. We also analyze Mono-Amine Oxidase (MAO) and Tyrosine Hydroxylase (TH) enzymatic activity. The results demonstrate that compared to SF, AB is more responsive to both acute alcohol exposure and acute alcohol withdrawal at the level of neurochemistry, a finding that correlates well with prior behavioural observations and one which suggests the involvement of genes in the observed alcohol effects. We discuss correlations between the current results and prior behavioural findings, and stress the importance of characterization of zebrafish strains for future behaviour genetic and psychopharmacology studies.
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影响因子：
11.2
作者：
GLASS, GV;PECKHAM, PD;SANDERS, JR
通讯作者：
SANDERS, JR
影响因子：
2.9
作者：
Buske, Christine;Gerlai, Robert
通讯作者：
Gerlai, Robert
影响因子：
2.7
作者：
Pan, Yi;Kaiguo, Mo;Gerlai, Robert
通讯作者：
Gerlai, Robert
影响因子：
2.3
作者：
Maccioni, Paola;Colombo, Giancarlo
通讯作者：
Colombo, Giancarlo
DOI：
10.1139/o67-185
发表时间：
1967-01-01
期刊：
CANADIAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
MCGEER, EG;GIBSON, S;MCGEER, PL
通讯作者：
MCGEER, PL