Early embryonic ethanol exposure impairs shoaling and the dopaminergic and serotoninergic systems in adult zebrafish.
Early embryonic ethanol exposure impairs shoaling and the dopaminergic and serotoninergic systems in adult zebrafish.
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DOI：
10.1016/j.ntt.2011.05.009
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发表时间：
2011-11
影响因子：
2.9
通讯作者：
Gerlai, Robert
中科院分区：
文献类型：
--
作者：
Buske, Christine;Gerlai, Robert
胎儿酒精综合症（FAS）是一种毁灭性的疾病，伴有多种形态和行为异常。人类 FAS 已在包括斑马鱼在内的实验动物中建立模型。最近，胚胎暴露于低剂量乙醇已被证明会损害斑马鱼的行为，但没有任何明显的形态变化。暴露的斑马鱼对动画同种图像的反应减弱。然而，尚未在真实的浅滩中研究胚胎乙醇暴露的影响，并且行为障碍的潜在机制也未知。在这里，我们发现，受精后 70 天测量时，受精后 24 小时将斑马鱼浸泡在 0.25% 和 0.50%（体积/体积）酒精中 2 小时，可显着增加自由游动斑马鱼群体成员之间的距离。我们还表明，这种行为受损伴随着多巴胺、DOPAC、血清素和 5HIAA 水平的降低（通过全脑提取物的 HPLC 定量）。我们的结果表明，在斑马鱼的发育过程中，即使短时间内使用非常低浓度的酒精也会损害其行为和大脑功能。我们认为观察到的行为障碍不太可能是由于表现能力的改变造成的，例如运动功能或感知，但可能与社会行为本身有关。我们还认为，我们的神经化学数据代表了理解斑马鱼这种异常机制的第一步，这可能会导致更好的人类 FAS 建模，并最终可能带来更好的治疗方法。
Fetal Alcohol Syndrome (FAS) is a devastating disorder accompanied by numerous morphological and behavioral abnormalities. Human FAS has been modeled in laboratory animals including the zebrafish. Recently, embryonic exposure to low doses of ethanol has been shown to impair behavior without any gross morphological alterations in zebrafish. The exposed zebrafish showed reduced responses to animated conspecific images. The effect of embryonic ethanol exposure, however, has not been investigated in a real shoal and the potential mechanisms underlying the behavioral impairment are also unknown. Here we show that a 2 hour long immersion in 0.25% and 0.50% (vol/vol) alcohol at 24 hours post fertilization significantly increases the distance among members of freely swimming groups of zebrafish when measured at 70 days post fertilization. We also show that this impaired behavior is accompanied by reduced levels of dopamine, DOPAC, serotonin and 5HIAA as quantified by HPLC from whole brain extracts. Our results demonstrate that even very low concentrations of alcohol applied for a short period of time during the development of zebrafish can impair behavior and brain function. We argue that the observed behavioral impairment is not likely to be due to altered performance capabilities, e.g. motor function or perception, but possibly to social behavior itself. We also argue that our neurochemical data represent the first step towards understanding the mechanisms of this abnormality in zebrafish, which may lead to better modeling of, and ultimately perhaps better therapies for human FAS.
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影响因子：
2.9
作者：
Bilotta, J;Barnett, JA;Saszik, S
通讯作者：
Saszik, S
DOI：
10.1007/bf00612563
发表时间：
1981-01-01
期刊：
JOURNAL OF COMPARATIVE PHYSIOLOGY
影响因子：
--
作者：
PARTRIDGE, BL
通讯作者：
PARTRIDGE, BL
影响因子：
2.7
作者：
Miller, Noam;Gerlai, Robert
通讯作者：
Gerlai, Robert
影响因子：
2.7
作者：
Al-Imari, Lina;Gerlai, Robert
通讯作者：
Gerlai, Robert
影响因子：
15.9
作者：
KUHAR, MJ;RITZ, MC;BOJA, JW
通讯作者：
BOJA, JW