De novo sequencing, assembly and characterization of antennal transcriptome of Anomala corpulenta Motschulsky (Coleoptera: Rutelidae).
De novo sequencing, assembly and characterization of antennal transcriptome of Anomala corpulenta Motschulsky (Coleoptera: Rutelidae).
复制标题
DOI：
10.1371/journal.pone.0114238
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Su W
中科院分区：
文献类型：
--
作者：
Chen H;Lin L;Xie M;Zhang G;Su W
铜绿丽金龟是一种重要的害虫，在农业、园艺和林业上造成巨大的经济损失。在我国分布广泛，幼虫和成虫均可造成严重危害。由于幼虫生活在地下，所以很难控制这种害虫。任何新的控制战略都应利用大量和经常使用的化学杀虫剂的替代品。然而，对A.由于缺乏基因组资源，利用新一代测序技术可以在短时间内、低成本地获得基因组资源。在这项研究中，我们进行了从头测序，组装和表征的触角转录组的A。肥大的。利用Illumina测序技术对A.肥大的。获得约7670万个总原始读数和约6890万个总干净读数，然后组装35，656个单基因。在这些unigenes中，有21，463个可以在NCBI nr数据库中注释，并且在注释的unigenes中，分别有11，154和6，625个unigenes可以指定为GO和COG。此外，16，350个unigenes可以在Swiss-Prot数据库中注释，14，499个unigenes可以映射到KEGG Pathway数据库中的258个通路上。我们还发现了24个与OBP相关的unigenes，6个与CSP相关的unigenes，总共167个与化学检测相关的unigenes。我们分析了4个OBP和3个CSP序列，其RT-qPCR结果与其FPKM值吻合良好。我们首次大规模制备了A. corpulenta，这是一个在公共数据库中几乎没有基因组信息的物种。这些化学检测特异基因的发现将促进对A.肥大的。这些结果为A.肥大的。
Anomala corpulenta is an important insect pest and can cause enormous economic losses in agriculture, horticulture and forestry. It is widely distributed in China, and both larvae and adults can cause serious damage. It is difficult to control this pest because the larvae live underground. Any new control strategy should exploit alternatives to heavily and frequently used chemical insecticides. However, little genetic research has been carried out on A. corpulenta due to the lack of genomic resources. Genomic resources could be produced by next generation sequencing technologies with low cost and in a short time. In this study, we performed de novo sequencing, assembly and characterization of the antennal transcriptome of A. corpulenta. Illumina sequencing technology was used to sequence the antennal transcriptome of A. corpulenta. Approximately 76.7 million total raw reads and about 68.9 million total clean reads were obtained, and then 35,656 unigenes were assembled. Of these unigenes, 21,463 of them could be annotated in the NCBI nr database, and, among the annotated unigenes, 11,154 and 6,625 unigenes could be assigned to GO and COG, respectively. Additionally, 16,350 unigenes could be annotated in the Swiss-Prot database, and 14,499 unigenes could map onto 258 pathways in the KEGG Pathway database. We also found 24 unigenes related to OBPs, 6 to CSPs, and in total 167 unigenes related to chemodetection. We analyzed 4 OBPs and 3CSPs sequences and their RT-qPCR results agreed well with their FPKM values. We produced the first large-scale antennal transcriptome of A. corpulenta, which is a species that has little genomic information in public databases. The identified chemodetection unigenes can promote the molecular mechanistic study of behavior in A. corpulenta. These findings provide a general sequence resource for molecular genetics research on A. corpulenta.
登录
查看更多内容
影响因子：
4.4
作者：
Feldmeyer B;Wheat CW;Krezdorn N;Rotter B;Pfenninger M
通讯作者：
Pfenninger M
影响因子：
2.8
作者：
Huang YW;Huang TH;Wang LS
通讯作者：
Wang LS
DOI：
10.1046/j.1432-1327.1999.00438.x
发表时间：
1999-06-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Angeli, S;Ceron, F;Pelosi, P
通讯作者：
Pelosi, P
影响因子：
3.7
作者：
Mikheyev AS;Vo T;Wee B;Singer MC;Parmesan C
通讯作者：
Parmesan C
圣甲虫Holotrichia oblita Faldermann（鞘翅目：金龟科）嗅觉感受器中两种新型气味结合蛋白的功能和免疫细胞化学定位
影响因子：
3.5
作者：
Deng, Sisi;Yin, Jiao;Li, Kebin
通讯作者：
Li, Kebin