L-selectin can mediate leukocyte rolling in untreated mesenteric venules in vivo independent of E- or P-selectin
L-selectin can mediate leukocyte rolling in untreated mesenteric venules in vivo independent of E- or P-selectin
复制标题
L-选择素可以在体内介导未经处理的肠系膜微静脉中的白细胞滚动，不依赖于E-或P-选择素
DOI：
10.1182/blood.v82.5.1632.1632
复制
发表时间：
1993
期刊：
影响因子：
20.3
通讯作者：
G. Kansas
中科院分区：
文献类型：
--
作者：
K. Ley;T. Tedder;G. Kansas
粒细胞在急性炎症反应中的募集是由它们沿着毛细血管后小静脉的内皮衬里滚动而开始的。为了确定单独表达L-选择素是否足以进行滚动，将人L-选择素基因导入不结合E-或P-选择素的小鼠前B淋巴细胞系300.19，使其稳定表达L-选择素，其表达水平与血淋巴细胞相似。将荧光标记细胞逆行注入麻醉大鼠肠系膜上动脉的侧支。在肠系膜小静脉中，白细胞在没有故意刺激的情况下发生滚动。平均而言，17%+/-6%的L-选择素转染体在观察到的小静脉中卷曲，而模拟转染组的细胞不卷曲。L-选择素转染体在手术后约20分钟开始滚动，并持续至少120分钟。相反，HL-60早幼粒细胞表达唾液酸-刘易斯(X)四糖(SLE(X))，但不表达L-选择素，并且在体外与E-和P-选择素结合，在20-120分钟内不滚动，但一些HL-60细胞在非常早的(2小时)时间点滚动。用识别L-选择素凝集素结构域的两种功能阻断单抗中的任何一种预先处理，均可完全阻断L-选择素转染体的滚动，并显著减少(70%)分离的人粒细胞的滚动。综上所述，这些结果表明L-选择素可能通过其凝集素活性来调节白细胞的滚动。
Granulocyte recruitment during the acute inflammatory response is initiated by their rolling along the endothelial lining of postcapillary venules. To determine whether expression of L-selectin alone is sufficient for rolling, the murine pre-B lymphocytic cell line 300.19, which does not bind E- or P-selectin, was transfected with human L-selectin cDNA, which led to stable L-selectin expression at a level similar to that of blood lymphocytes. Fluorescent-labeled cells were infused retrogradely into a side branch of the superior mesenteric artery of anesthetized rats. In venules of the mesenteric membrane, leukocyte rolling occurs without intentional stimulation. On average, 17% +/- 6% of L-selectin transfectants rolled in the observed venules, while mock-transfected cells did not roll. Rolling of L-selectin transfectants began approximately 20 minutes after surgery and continued for at least 120 minutes. In contrast, HL-60 promyelocytes, which express sialyl-Lewis(x) tetrasaccharide (sLe(x)) but not L-selectin and that bind to E- and P-selectin in vitro, did not roll between 20 and 120 minutes, but some HL-60 cells rolled at very early ( 2 hours) time points. Pretreatment with either of two function-blocking monoclonal antibodies recognizing the lectin domain of L-selectin completely blocked rolling of L-selectin transfectants and sharply reduced (by 70%) rolling of isolated human granulocytes. Taken together, these results show that L-selectin can mediate leukocyte rolling by virtue of its lectin activity.
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影响因子：
4.4
作者：
T. Tedder;Ann C. Penta;H. Levine;A. Freedman
通讯作者：
T. Tedder;Ann C. Penta;H. Levine;A. Freedman
DOI：
10.1073/pnas.88.17.7538
发表时间：
1991-09-01
影响因子：
11.1
作者：
VONANDRIAN, UH;CHAMBERS, JD;BUTCHER, EC
通讯作者：
BUTCHER, EC
影响因子：
56.9
作者：
BEVILACQUA, MP;STENGELIN, S;SEED, B
通讯作者：
SEED, B
影响因子：
20.3
作者：
G. Schmid-Schönbein;Y. Y. Shih-Y.;S. Chien
通讯作者：
G. Schmid-Schönbein;Y. Y. Shih-Y.;S. Chien
影响因子：
11.2
作者：
K. Fukushima;M. Hirota;P. Terasaki;A. Wakisaka;H. Togashi;D. Chia;N. Suyama;Y. Fukushi;E. Nudelman;S. Hakomori
通讯作者：
K. Fukushima;M. Hirota;P. Terasaki;A. Wakisaka;H. Togashi;D. Chia;N. Suyama;Y. Fukushi;E. Nudelman;S. Hakomori