Fgf8 regulates first pharyngeal arch segmentation through pouch-cleft interactions.
Fgf8 regulates first pharyngeal arch segmentation through pouch-cleft interactions.
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DOI：
10.3389/fcell.2023.1186526
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发表时间：
2023
影响因子：
5.5
通讯作者：
中科院分区：
文献类型：
--
作者：
简介：咽弓是短暂的发育结构，在脊椎动物中，引起头部和颈部的组织。一个关键的过程中，潜在的规范不同的弓衍生物是分割的弓沿着前后轴。外胚层-内胚层界面的形成是这一过程的关键介质，虽然它是必不可少的，但调节这些界面建立的机制在囊袋之间和分类群之间各不相同。 研究方法：在这里，我们专注于与第一咽弓，第一咽袋（pp 1）和第一咽裂（pc 1），和FGF 8剂量在这些过程中的作用，在小鼠模型系统的上皮细胞的图案和形态发生。 结果：我们发现Fgf 8水平的严重降低会破坏pp 1和pc 1的发育。值得注意的是，pp 1的外袋化对于Fgf 8的减少在很大程度上是稳健的，然而，当Fgf 8低时，pp 1沿沿着近端-远端轴的延伸失败。我们的数据表明，FGF 8是需要规范的区域身份在pp 1和pc 1，在细胞极性的局部变化，并延长和延伸的pp 1和pc 1。 讨论内容：基于FGF 8介导的组织之间的关系pp 1和pc 1的变化，我们假设，pp 1的扩展需要与pc 1的物理相互作用。总的来说，我们的数据表明，侧表面外胚层在分割第一咽弓，以前被低估的关键作用。
Introduction: The pharyngeal arches are transient developmental structures that, in vertebrates, give rise to tissues of the head and neck. A critical process underlying the specification of distinct arch derivatives is segmentation of the arches along the anterior-posterior axis. Formation of ectodermal-endodermal interfaces is a key mediator of this process, and although it is essential, mechanisms regulating the establishment of these interfaces vary between pouches and between taxa. Methods: Here, we focus on the patterning and morphogenesis of epithelia associated with the first pharyngeal arch, the first pharyngeal pouch (pp1) and the first pharyngeal cleft (pc1), and the role of Fgf8 dosage in these processes in the mouse model system. Results: We find that severe reductions of Fgf8 levels disrupt both pp1 and pc1 development. Notably, out-pocketing of pp1 is largely robust to Fgf8 reductions, however, pp1 extension along the proximal-distal axis fails when Fgf8 is low. Our data indicate that Fgf8 is required for specification of regional identity in both pp1 and pc1, for localized changes in cell polarity, and for elongation and extension of both pp1 and pc1. Discussion: Based on Fgf8-mediated changes in tissue relationships between pp1 and pc1, we hypothesize that extension of pp1 requires physical interaction with pc1. Overall, our data indicate a critical role for the lateral surface ectoderm in segmentation of the first pharyngeal arch that has previously been under-appreciated.
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影响因子：
2.7
作者：
Griffin, John N.;Compagnucci, Claudia;Hu, Diane;Fish, Jennifer;Klein, Ophir;Marcucio, Ralph;Depew, Michael J.
通讯作者：
Depew, Michael J.
影响因子：
2.7
作者：
Edlund, Renee K.;Ohyama, Takahiro;Kantarci, Husniye;Riley, Bruce B.;Groves, Andrew K.
通讯作者：
Groves, Andrew K.
影响因子：
11.8
作者：
Choe, Chong Pyo;Collazo, Andres;Crump, J. Gage
通讯作者：
Crump, J. Gage
DOI：
10.1002/jez.b.21205
发表时间：
2008-06-15
影响因子：
2.2
作者：
Depew, Michael J.;Compagnucci, Claudia
通讯作者：
Compagnucci, Claudia
影响因子：
3
作者：
GOWER, JC
通讯作者：
GOWER, JC