Suppressive effects of vitamin C-treated induced-regulatory T cells on heart allograft rejection under vitamin C-deficient or -sufficient conditions.
Suppressive effects of vitamin C-treated induced-regulatory T cells on heart allograft rejection under vitamin C-deficient or -sufficient conditions.
复制标题
DOI：
10.1371/journal.pone.0246967
复制
发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Yang J
中科院分区：
文献类型：
--
作者：
Hwang JH;Piao H;Jang JY;Lee SK;Han D;Lee GM;Go C;Kim Y;Oh KI;Kang JS;Yan JJ;Yang J
维生素C处理的诱导调节性T细胞（V-iTcells）的Foxp 3稳定性上级常规iTcells（C-iTcells）。然而，在维生素C缺乏的条件下，如在人类中所见，V-iTdR在同种异体移植排斥反应中的作用仍不清楚。我们的目的是阐明维生素C治疗对gulo基因敲除（Gulo-KO）小鼠以及野生型（WT）小鼠iTclad的产生和维持的作用，以及V-iTclad对Gulo-KO或WT受体小鼠中心脏同种异体移植物排斥的体外和体内抑制作用。在WT和Gulo-KO小鼠中，C-和V-iTdR之间iTdR的转化效率相似。来自WT或Gulo-KO小鼠的V-iTdR显示出比C-iTdR更好的体外Foxp 3稳定性，尽管WT V-iTdR和Gulo-KO V-iTdR之间没有差异。此外，来自WT或Gulo-KO小鼠的V-iTdR比C-iTdR更好地抑制体外T细胞增殖。在过继转移C-或V-iTlR后，进行从BALB/c小鼠到WT或维生素C缺乏的Gulo-KO C57 BL/6 J小鼠的异养心脏移植。在WT和Gulo-KO小鼠中，V-iTdR和C-iTdR延长了心脏同种异体移植物的存活。然而，C-和V-iTreg组之间没有差异。补充低剂量或高剂量的维生素C并没有引起接受V-iTdR的Gulo-KO受体心脏移植物存活率的显著变化。总之，在WT或维生素C缺乏的受体中，V-iTdR对心脏移植物存活的抑制作用并不比C-iTdR好。
Foxp3 stability of vitamin C-treated induced-regulatory T cells (V-iTregs) is superior to that of conventional iTregs (C-iTregs). However, the role of V-iTregs in allograft rejection under vitamin C-deficient conditions, such as those seen in humans, remains unclear. We aimed to elucidate the role of vitamin C treatment on generation and maintenance of iTregs from gulo knockout (Gulo-KO) mice as well as wild type (WT) mice, and in vitro and in vivo suppressive effects of V-iTregs on heart allograft rejection in either Gulo-KO or WT recipient mice. Conversion efficiency of iTregs was similar between C- and V-iTregs in both WT and Gulo-KO mice. V-iTregs from WT or Gulo-KO mice showed better in vitro Foxp3 stability than C-iTregs, although there was no difference between WT V-iTregs and Gulo-KO V-iTregs. Furthermore, V-iTregs from WT or Gulo-KO mice suppressed in vitro T cell proliferation better than C-iTregs. Heterotrophic heart transplantation from BALB/c mice to WT or vitamin C-deficient Gulo-KO C57BL/6J mice was performed following adoptive transfer of C- or V-iTregs. V-iTregs as well as C-iTregs prolonged heart allograft survival in WT and Gulo-KO mice. However, there was no difference between the C- and V-iTreg groups. Supplementation of low- or high-dose vitamin C did not induce significant changes in heart allograft survival in Gulo-KO recipients that had received V-iTregs. In conclusion, V-iTregs do not exert better suppressive effects on heart allograft survival than C-iTregs in either WT or vitamin C-deficient recipients.
登录
查看更多内容
影响因子：
100.3
作者：
Huehn, Jochen;Polansky, Julia K.;Hamann, Alf
通讯作者：
Hamann, Alf
影响因子：
19.6
作者：
Koo, Tai Yeon;Lee, Jae-Ghi;Yang, Jaeseok
通讯作者：
Yang, Jaeseok
DOI：
10.1016/j.bbrc.2014.06.110
发表时间：
2014-07-18
影响因子：
3.1
作者：
Nair, Varun Sasidharan;Oh, Kwon Ik
通讯作者：
Oh, Kwon Ik
影响因子：
6
作者：
Kim H;Bae S;Yu Y;Kim Y;Kim HR;Hwang YI;Kang JS;Lee WJ
通讯作者：
Lee WJ
影响因子：
8.7
作者：
Shevach EM;Thornton AM
通讯作者：
Thornton AM