Genomic and Virological Characterization of SARS-CoV-2 Variants in a Subset of Unvaccinated and Vaccinated U.S. Military Personnel.
Genomic and Virological Characterization of SARS-CoV-2 Variants in a Subset of Unvaccinated and Vaccinated U.S. Military Personnel.
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DOI：
10.3389/fmed.2021.836658
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发表时间：
2021
影响因子：
3.9
通讯作者：
Bishop-Lilly KA
中科院分区：
文献类型：
--
作者：
Smith DR;Singh C;Green J;Lueder MR;Arnold CE;Voegtly LJ;Long KA;Rice GK;Luquette AE;Miner HL;Glang L;Bennett AJ;Miller RH;Malagon F;Cer RZ;Bishop-Lilly KA
SARS-CoV-2变种的出现使控制COVID-19大流行的努力变得复杂。加强对SARS-CoV-2的基因组监测对于早期发现新出现的变异、追踪变异的运动和监测对策的有效性至关重要。此外，确定临床样本中存在的活病毒量有助于更好地了解这些变异对病毒脱落的影响。在这项研究中，我们分析了2020年3月至2021年11月初从美国（U.S.）作为国防卫生局（DHA）全球新发感染监测（GEIS）计划的一部分，驻扎在世界各地的军事人员和医疗保健系统受益人。SARS-CoV-2定量真实的时间反转录PCR（qRT-PCR）阳性样本通过下一代测序进行表征，并对一个子集进行分析，以分离和定量活病毒。毫不奇怪，我们发现Delta变异株是2021年7月开始在美国军事人员中流行的主要毒株，主要代表疫苗突破性感染（VBIs）病例。在VBI中，我们发现与涉及其他变体的病例相比，Delta变体病例的鼻拭子样本中的活病毒增加了50倍。值得注意的是，我们发现，与在获得批准疫苗之前感染其他变体的未接种疫苗人员相比，来自涉及Delta的VBI的鼻拭子样本中的活病毒增加了40倍。这项研究提供了重要的见解，从一个独特的研究人群与全球存在的SARS-CoV-2分离株的基因组和病毒学特征。
The emergence of SARS-CoV-2 variants complicates efforts to control the COVID-19 pandemic. Increasing genomic surveillance of SARS-CoV-2 is imperative for early detection of emerging variants, to trace the movement of variants, and to monitor effectiveness of countermeasures. Additionally, determining the amount of viable virus present in clinical samples is helpful to better understand the impact these variants have on viral shedding. In this study, we analyzed nasal swab samples collected between March 2020 and early November 2021 from a cohort of United States (U.S.) military personnel and healthcare system beneficiaries stationed worldwide as a part of the Defense Health Agency's (DHA) Global Emerging Infections Surveillance (GEIS) program. SARS-CoV-2 quantitative real time reverse-transcription PCR (qRT-PCR) positive samples were characterized by next-generation sequencing and a subset was analyzed for isolation and quantification of viable virus. Not surprisingly, we found that the Delta variant is the predominant strain circulating among U.S. military personnel beginning in July 2021 and primarily represents cases of vaccine breakthrough infections (VBIs). Among VBIs, we found a 50-fold increase in viable virus in nasal swab samples from Delta variant cases when compared to cases involving other variants. Notably, we found a 40-fold increase in viable virus in nasal swab samples from VBIs involving Delta as compared to unvaccinated personnel infected with other variants prior to the availability of approved vaccines. This study provides important insight about the genomic and virological characterization of SARS-CoV-2 isolates from a unique study population with a global presence.
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DOI：
10.1016/s1473-3099(21)00475-8
发表时间：
2022-01
期刊：
The Lancet. Infectious diseases
影响因子：
--
作者：
Twohig KA;Nyberg T;Zaidi A;Thelwall S;Sinnathamby MA;Aliabadi S;Seaman SR;Harris RJ;Hope R;Lopez-Bernal J;Gallagher E;Charlett A;De Angelis D;Presanis AM;Dabrera G;COVID-19 Genomics UK (COG-UK) consortium
通讯作者：
COVID-19 Genomics UK (COG-UK) consortium
SARS-COV-2血统的估计可传播和影响B.1.1.7在英国。
DOI：
10.1126/science.abg3055
发表时间：
2021-04-09
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Davies NG;Abbott S;Barnard RC;Jarvis CI;Kucharski AJ;Munday JD;Pearson CAB;Russell TW;Tully DC;Washburne AD;Wenseleers T;Gimma A;Waites W;Wong KLM;van Zandvoort K;Silverman JD;CMMID COVID-19 Working Group;COVID-19 Genomics UK (COG-UK) Consortium;Diaz-Ordaz K;Keogh R;Eggo RM;Funk S;Jit M;Atkins KE;Edmunds WJ
通讯作者：
Edmunds WJ
影响因子：
16.6
作者：
Li B;Deng A;Li K;Hu Y;Li Z;Shi Y;Xiong Q;Liu Z;Guo Q;Zou L;Zhang H;Zhang M;Ouyang F;Su J;Su W;Xu J;Lin H;Sun J;Peng J;Jiang H;Zhou P;Hu T;Luo M;Zhang Y;Zheng H;Xiao J;Liu T;Tan M;Che R;Zeng H;Zheng Z;Huang Y;Yu J;Yi L;Wu J;Chen J;Zhong H;Deng X;Kang M;Pybus OG;Hall M;Lythgoe KA;Li Y;Yuan J;He J;Lu J
通讯作者：
Lu J
DOI：
10.1093/cid/ciab986
发表时间：
2022-08-24
期刊：
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
影响因子：
--
作者：
Huai Luo C;Paul Morris C;Sachithanandham J;Amadi A;Gaston DC;Li M;Swanson NJ;Schwartz M;Klein EY;Pekosz A;Mostafa HH
通讯作者：
Mostafa HH
影响因子：
3.7
作者：
Bushnell B;Rood J;Singer E
通讯作者：
Singer E