Tiling among stereotyped dendritic branches in an identified Drosophila motoneuron.
Tiling among stereotyped dendritic branches in an identified Drosophila motoneuron.
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DOI：
10.1002/cne.22380
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发表时间：
2010-06-15
影响因子：
2.5
通讯作者：
Duch, C.
中科院分区：
文献类型：
--
作者：
Vonhoff, F.;Duch, C.
不同类型的神经元可以通过其树突的特定目标位置和分支模式来区分，这些树突构成了大脑连接的蓝图。阐明哪些特定信号控制树突结构的不同方面，例如分支和伸长、修剪和生长停止、区域形成、平铺和自我回避，需要对控制神经元和基因操纵神经元进行定量比较。单独识别的果蝇神经元的高度保守的形状使它们非常适合树突结构原理的分析。然而，迄今为止，尚不清楚已确定的中枢神经元的树突结构原理受到如何严格的调节。这项研究使用了已鉴定的果蝇飞行运动神经元 (MN5) 的树突结构的定量重建，该神经元具有复杂的树突树，包括 4,000 多个树突分支，总长度为 6 毫米。 MN5 包含固定数量的 23 个树突子树，它们平铺成不同的、不重叠的弥散运动神经元体积。跨动物比较和定量分析表明，同一神经元的不同树突子树的平铺是由子树之间的竞争和排斥相互作用引起的，可能允许不同的树突区室连接到不同的电路元件。我们还表明，不同野生型和 GAL4 驱动蝇系之间的树突结构相似。公制和拓扑树突结构特征足够恒定，可以通过遗传操作研究潜在的控制机制。树突区域和某些拓扑测量（例如树的致密性）是最恒定的，这表明它们反映了神经元的内在分子身份。
Different types of neurons can be distinguished by the specific targeting locations and branching patterns of their dendrites, which form the blueprint for wiring the brain. Unraveling which specific signals control different aspects of dendritic architecture, such as branching and elongation, pruning and cessation of growth, territory formation, tiling, and self-avoidance requires a quantitative comparison in control and genetically manipulated neurons. The highly conserved shapes of individually identified Drosophila neurons make them well suited for the analysis of dendritic architecture principles. However, to date it remains unclear how tightly dendritic architecture principles of identified central neurons are regulated. This study uses quantitative reconstructions of dendritic architecture of an identified Drosophila flight motoneuron (MN5) with a complex dendritic tree, comprising more than 4,000 dendritic branches and 6 mm total length. MN5 contains a fixed number of 23 dendritic subtrees, which tile into distinct, nonoverlapping volumes of the diffuse motor neuropil. Across-animal comparison and quantitative analysis suggest that tiling of the different dendritic subtrees of the same neuron is caused by competitive and repulsive interactions among subtrees, perhaps allowing different dendritic compartments to be connected to different circuit elements. We also show that dendritic architecture is similar among different wildtype and GAL4 driver fly lines. Metric and topological dendritic architecture features are sufficiently constant to allow for studies of the underlying control mechanisms by genetic manipulations. Dendritic territory and certain topological measures, such as tree compactness, are most constant, suggesting that these reflect the intrinsic molecular identity of the neuron.
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影响因子：
16.2
作者：
Gao, FB;Kohwi, M;Jan, YN
通讯作者：
Jan, YN
影响因子：
5.3
作者：
Duch, C;Levine, RB
通讯作者：
Levine, RB
影响因子：
2.5
作者：
IKEDA, K;KOENIG, JH
通讯作者：
KOENIG, JH
DOI：
10.1002/neu.20143
发表时间：
2005-07-01
期刊：
JOURNAL OF NEUROBIOLOGY
影响因子：
--
作者：
Cooke, BM;Woolley, CS
通讯作者：
Woolley, CS
影响因子：
5.3
作者：
Duch, C;Mentel, T
通讯作者：
Mentel, T