Gr64f is required in combination with other gustatory receptors for sugar detection in Drosophila.
Gr64f is required in combination with other gustatory receptors for sugar detection in Drosophila.
复制标题
DOI：
10.1016/j.cub.2008.10.009
复制
发表时间：
2008-11-25
期刊：
影响因子：
9.2
通讯作者：
Montell, Craig
中科院分区：
文献类型：
--
作者：
Jiao, Yuchen;Moon, Seok Jun;Wang, Xiaoyue;Ren, Qiuting;Montell, Craig
果蝇，果蝇，黑腹果蝇，检测甜味化合物的机制知之甚少；然而，68个味觉受体(GRs)家族中的一个子集已经成为关键受体。这七个跨膜受体包括Gr5a和Gr64簇(Gr64a)中六个基因中的至少一个成员，它们在糖反应神经元中表达。Gr5a的突变阻止了海藻糖的检测，而Gr64a的突变则削弱了对蔗糖、麦芽糖和葡萄糖的反应。最近的研究表明，这些糖受体可能需要一个辅助受体才能在体内发挥作用；然而，假定的辅助受体的身份尚不清楚。在目前的工作中，我们证明了Gr64f和Gr5a联合使用对于海藻糖的行为反应和由于应用海藻糖而产生的动作电位是必需的。Gr64f还需要与Gr64a一起使用，以弥补由于整个Gr64簇缺失而导致的对蔗糖、麦芽糖和葡萄糖敏感性的缺陷。这些数据表明，果蝇的糖受体具有多聚体的功能，而Gr64f在检测糖的过程中广泛需要作为辅助受体。
The mechanisms by which the fruitfly, Drosophila melanogaster, detect sweet compounds are poorly understood; however, a subset of the family of 68 gustatory receptors (Grs) has emerged as the key receptors. These seven transmembrane receptors include Gr5a and at least one member of the six genes in the Gr64 cluster (Gr64a), which are expressed in sugar-responsive neurons. Disruption of Gr5a prevents the detection of trehalose, while mutation of Gr64a impairs the responses to sucrose, maltose and glucose. Recent studies suggest that these sugar receptors may require a co-receptor for function in vivo; however, the identity of the putative co-receptor is not known. In the current work, we demonstrate that Gr64f is required in combination with Gr5a for the behavioral response to trehalose and for production of action potentials due to application of trehalose. Gr64f was also required in concert with Gr64a to rescue the defects in the sensitivities to sucrose, maltose and glucose, resulting from deletion of the entire Gr64 cluster. These data suggest that Drosophila sugar receptors function as multimers and that Gr64f is required broadly as a co-receptor for the detection of sugars.
登录
查看更多内容
影响因子：
64.8
作者：
Wicher, Dieter;Schaefer, Ronny;Hansson, Bill S.
通讯作者：
Hansson, Bill S.
影响因子：
3.3
作者：
Metaxakis, A;Oehler, S;Savakis, C
通讯作者：
Savakis, C
影响因子：
9.2
作者：
Ueno, K;Ohta, M;Isono, K
通讯作者：
Isono, K
影响因子：
16.2
作者：
Larsson, MC;Domingos, AI;Vosshall, LB
通讯作者：
Vosshall, LB
影响因子：
64.5
作者：
Wang, ZR;Singhvi, A;Scott, K
通讯作者：
Scott, K