Bone marrow mesenchymal stem cell-derived exosomes promote rotator cuff tendon-bone healing by promoting angiogenesis and regulating M1 macrophages in rats.
Bone marrow mesenchymal stem cell-derived exosomes promote rotator cuff tendon-bone healing by promoting angiogenesis and regulating M1 macrophages in rats.
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骨髓间充质干细胞来源的外切体通过促进血管生成和调节M1巨噬细胞促进大鼠肩袖肌腱-骨愈合。
DOI：
10.1186/s13287-020-02005-x
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发表时间：
2020-11-25
影响因子：
7.5
通讯作者：
Sun L
中科院分区：
文献类型：
--
作者：
Huang Y;He B;Wang L;Yuan B;Shu H;Zhang F;Sun L
肩袖撕裂（RCT）通常需要重建手术。肌腱-骨愈合是肩袖重建术的关键，但肌腱-骨愈合过程复杂且困难。骨髓间充质干细胞（Mesenchymal stem cells，MSCs）被认为是促进肌腱-骨愈合的有效方法。间充质干细胞具有强大的旁分泌、抗炎、免疫调节和血管生成潜力。最近的研究表明，MSC通过exosomes实现许多调节功能。本研究旨在探讨骨髓间充质干细胞来源的外泌体（BMSC-Exos）在肌腱-骨愈合中的作用。我们的研究发现BMSC-Exos促进人脐静脉内皮细胞（HUVECs）的增殖、迁移和血管生成管形成。BMSC-Exos实现这一点的机制可能是通过调节血管生成信号通路。此外，BMSC-Exos可抑制M1巨噬细胞的极化，抑制M1巨噬细胞分泌促炎因子。大鼠肩袖重建后，尾静脉注射BMSC-Exos，分析其对肩袖肌腱-骨界面愈合的影响。证实BMSC-Exos增加了大鼠重建后肩袖的断裂载荷和刚度，诱导肩袖端点周围的血管生成，并促进肌腱-骨界面的生长。BMSC-Exos通过促进血管生成和抑制炎症促进大鼠肩袖重建后的腱-骨愈合。在线版本包含补充材料，可通过10.1186/s13287-020-02005-x获得。
Rotator cuff tears (RCTs) often require reconstructive surgery. Tendon-bone healing is critical for the outcome of rotator cuff reconstruction, but the process of tendon-bone healing is complex and difficult. Mesenchymal stem cells (MSCs) are considered to be an effective method to promote tendon-bone healing. MSCs have strong paracrine, anti-inflammatory, immunoregulatory, and angiogenic potential. Recent studies have shown that MSCs achieve many regulatory functions through exosomes. The purpose of this study was to explore the role of bone marrow mesenchymal stem cell-derived exosomes (BMSC-Exos) in tendon-bone healing. Our study found that BMSC-Exos promote the proliferation, migration, and angiogenic tube formation of human umbilical vein endothelial cells (HUVECs). The mechanism by which BMSC-Exos achieve this may be through the regulation of the angiogenic signaling pathway. In addition, BMSC-Exos can inhibit the polarization of M1 macrophages and inhibit the secretion of proinflammatory factors by M1 macrophages. After rotator cuff reconstruction in rats, BMSC-Exos were injected into the tail vein to analyze their effect on the rotator cuff tendon-bone interface healing. It was confirmed that BMSC-Exos increased the breaking load and stiffness of the rotator cuff after reconstruction in rats, induced angiogenesis around the rotator cuff endpoint, and promoted growth of the tendon-bone interface. BMSC-Exos promote tendon-bone healing after rotator cuff reconstruction in rats by promoting angiogenesis and inhibiting inflammation. The online version contains supplementary material available at 10.1186/s13287-020-02005-x.
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影响因子：
3.8
作者：
Ellera Gomes, Joao L.;da Silva, Ricardo Canquerini;Silla, Lucia M. R.;Abreu, Marcelo R.;Pellanda, Roberto
通讯作者：
Pellanda, Roberto
影响因子：
2.8
作者：
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通讯作者：
Thomopoulos, S
影响因子：
2.4
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通讯作者：
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影响因子：
4.8
作者：
Frank, Joshua B.;ElAttrache, Neal S.;Tibone, James E.
通讯作者：
Tibone, James E.
影响因子：
16.6
作者：
Azad T;Janse van Rensburg HJ;Lightbody ED;Neveu B;Champagne A;Ghaffari A;Kay VR;Hao Y;Shen H;Yeung B;Croy BA;Guan KL;Pouliot F;Zhang J;Nicol CJB;Yang X
通讯作者：
Yang X