NSSR1 is regulated by testosterone in the mouse uterus and extensively expressed in endometrial carcinoma
NSSR1 is regulated by testosterone in the mouse uterus and extensively expressed in endometrial carcinoma
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NSSR1 在小鼠子宫中受睾酮调节，并在子宫内膜癌中广泛表达
DOI：
10.1007/s13277-010-0128-3
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发表时间：
2011-04
期刊：
影响因子：
--
通讯作者：
Xu, Ping
中科院分区：
文献类型：
--
作者：
Peng, Zheng-Yu;Xiao, Ping-Jie;Qi, Yao;Zhang, Wei;Chen, Xian-Hua;Xu, Ping
神经显着丝氨酸/丝氨酸丰富蛋白1（NSSR 1）已被发现在抑制热休克和有丝分裂过程中的选择性剪接中发挥重要作用，并主要在神经组织中表达，如大脑神经元，小脑浦肯野细胞和视网膜双极细胞。最近，NSSR 1也被证明在睾丸中高表达，提示其在生殖系统中的潜在作用。在这份报告中，NSSR 1在小鼠子宫发育过程中的子宫内膜和腺上皮的柱状上皮中的表达，在成年小鼠子宫中睾酮对NSSR 1水平的调节，以及NSSR 1在人子宫内膜癌和卵巢癌中的表达水平，通过定量实时聚合酶链反应（qRT-PCR），蛋白质印迹和免疫组织化学进行了分析。我们证明了NSSR 1的表达在小鼠子宫内膜的柱状上皮和腺上皮中受到发育调节。此外，小鼠子宫中的NSSR 1水平由睾酮维持和调节。有趣的是，在人子宫内膜癌和卵巢癌中均观察到NSSR 1水平升高。我们的研究结果表明，NSSR 1的表达和分布是发育和生殖调控和上调子宫内膜癌以及卵巢癌，表明其在子宫发育和肿瘤发生的潜在参与。
Neural salient serine/arginine-rich protein 1 (NSSR1) has been found to play important roles in inhibiting alternative splicing during heat shock and mitosis and is predominantly expressed in neural tissues such as cerebral neurons, cerebellar Purkinje cells and bipolar cells of the retina. Recently, NSSR1 has also been shown to be highly expressed in the testes, suggesting its potential roles in reproductive system. In this report, the expression of NSSR1 in the columnar epithelium of the endometrium and gland epithelium during the development of the mouse uterus, the regulation of NSSR1 level by testosterone in the adult mouse uterus, and expression level of NSSR1 in both human endometrial carcinomas and ovarian cancers were analyzed by quantitative real-time polymerase chain reaction (qRT-PCR), Western blot, and immunohistochemistry. We demonstrated that the expression of NSSR1 was developmentally regulated in the columnar epithelium of the endometrium and gland epithelium in the mouse uterus. Additionally, the NSSR1 level in the mouse uterus was maintained and regulated by testosterone. Interestingly, an enhanced level of NSSR1 was observed in both human endometrial carcinomas and ovarian cancers. Our results suggest that expression and distribution of NSSR1 is developmentally and hormonally regulated and up-regulated in endometrial carcinomas as well as ovarian cancers, indicating its potential involvement in uterine development and tumorgenesis.
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影响因子：
--
作者：
G. Flouriot;C. Griffin;M. Kenealy;V. Sonntag-Buck;F. Gannon
通讯作者：
G. Flouriot;C. Griffin;M. Kenealy;V. Sonntag-Buck;F. Gannon
DOI：
10.1016/s1357-2725(97)00011-3
发表时间：
1997-05
期刊：
The international journal of biochemistry & cell biology
影响因子：
--
作者：
H. Nandurkar;L. Robb;J. Nicholl;D. Hilton;G. Sutherland;C. Begley
通讯作者：
H. Nandurkar;L. Robb;J. Nicholl;D. Hilton;G. Sutherland;C. Begley
DOI：
--
发表时间：
--
期刊：
--
影响因子：
--
作者：
通讯作者：
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影响因子：
4.8
作者：
Dong, XS;Shylnova, O;Lye, SJ
通讯作者：
Lye, SJ
影响因子：
3.8
作者：
G. Nie;A. Hampton;G. Fu;Y.-X. Liu;J. Findlay;L. Salamonsen
通讯作者：
G. Nie;A. Hampton;G. Fu;Y.-X. Liu;J. Findlay;L. Salamonsen