Endothelial cells expressing low levels of CD143 (ACE) exhibit enhanced sprouting and potency in relieving tissue ischemia.
Endothelial cells expressing low levels of CD143 (ACE) exhibit enhanced sprouting and potency in relieving tissue ischemia.
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DOI：
10.1007/s10456-014-9414-9
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发表时间：
2014-07
期刊：
影响因子：
9.8
通讯作者：
Mooney, David J.
中科院分区：
文献类型：
--
作者：
Silva, Eduardo A.;Eseonu, Chikezie;Mooney, David J.
The sprouting of endothelial cells from pre-existing blood vessels represents a critical event in the angiogenesis cascade. However only a fraction of cultured or transplanted endothelial cells form new vessels. Moreover it is unclear if this results from a stochastic process or instead relates to certain endothelial cells having a greater angiogenic potential. This study investigated whether there exists a sub-population of cultured endothelial cells with enhanced angiogenic potency in vitro and in vivo. First, endothelial cells that participated in sprouting, and non-sprouting cells, were separately isolated from a 3D fibrin gel sprouting assay. Interestingly, the sprouting cells, when placed back into the same assay, displayed a 7-fold increase in the number of sprouts, as compared with control cells. Angiotensin converting enzyme (CD143) was significantly down regulated on sprouting cells, as compared to regular endothelial cells. A subset of endothelial cells with low CD143 expression was then prospectively isolated from an endothelial cell culture. Finally, these cells were found to have greater potency in alleviating local ischemia, and restoring regional blood perfusion when transplanted into ischemic hindlimbs, as compared with unsorted endothelial cells. In summary, this study indicates that low expression of CD143 can be used as a biomarker to identify an endothelial cell subpopulation that is more capable to drive neovascularization.
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影响因子：
20.1
作者：
Kohlstedt, Karin;Trouvain, Caroline;Fleming, Ingrid
通讯作者：
Fleming, Ingrid
DOI：
10.1007/978-1-4614-0899-4_16
发表时间：
2012-01-01
期刊：
NOTCH SIGNALING IN EMBRYOLOGY AND CANCER
影响因子：
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作者：
Kume, Tsutomu
通讯作者：
Kume, Tsutomu
影响因子：
10.4
作者：
Li, X.;Claesson-Welsh, L.
通讯作者：
Claesson-Welsh, L.
影响因子：
5.4
作者：
Blanco, Raquel;Gerhardt, Holger
通讯作者：
Gerhardt, Holger
分子机制和血管生成的临床应用。
影响因子：
64.8
作者：
Carmeliet, Peter;Jain, Rakesh K.
通讯作者：
Jain, Rakesh K.