Quantitative proteomics reveals the effect of protein glycosylation in soybean root under flooding stress.
Quantitative proteomics reveals the effect of protein glycosylation in soybean root under flooding stress.
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DOI：
10.3389/fpls.2014.00627
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发表时间：
2014
影响因子：
5.6
通讯作者：
Komatsu S
中科院分区：
文献类型：
--
作者：
Mustafa G;Komatsu S
淹水胁迫严重影响大豆的生长发育，对大豆的种植有负面影响。为了了解早期大豆的水淹响应机制，使用糖蛋白质组学技术。用淹水处理两天大的大豆2天，并收集根。在全球范围内，积累水平的糖蛋白，揭示了交叉反应与刀豆球蛋白A下降了2天的洪水胁迫。使用刀豆球蛋白A凝集素树脂从总蛋白提取物中富集糖蛋白，并使用无凝胶蛋白质组学技术进行分析。111个和69个糖蛋白，分别确定没有和2天的洪水胁迫。这些确定的糖蛋白的功能分类表明，蛋白质降解，细胞壁，糖酵解相关的蛋白质的积累水平增加，而压力相关的蛋白质减少淹水胁迫。此外，定位于分泌途径的糖蛋白的积累水平降低淹水胁迫。在23个常见的糖蛋白之间的控制和洪水条件下，过氧化物酶和糖基水解酶减少了2天的洪水胁迫。洪水胁迫下调了内质网蛋白和N-糖基化相关蛋白的mRNA表达水平。这些结果表明，洪水可能会对与应激和蛋白质降解相关的蛋白质的N-糖基化过程产生负面影响;然而，参与糖酵解的糖蛋白被激活。
Flooding stress has a negative impact on soybean cultivation because it severely impairs growth and development. To understand the flooding responsive mechanism in early stage soybeans, a glycoproteomic technique was used. Two-day-old soybeans were treated with flooding for 2 days and roots were collected. Globally, the accumulation level of glycoproteins, as revealed by cross-reaction with concanavalin A decreased by 2 days of flooding stress. Glycoproteins were enriched from total protein extracts using concanavalin A lectin resin and analyzed using a gel-free proteomic technique. One-hundred eleven and 69 glycoproteins were identified without and with 2 days of flooding stress, respectively. Functional categorization of these identified glycoproteins indicated that the accumulation level of proteins related to protein degradation, cell wall, and glycolysis increased, while stress-related proteins decreased under flooding stress. Also the accumulation level of glycoproteins localized in the secretory pathway decreased under flooding stress. Out of 23 common glycoproteins between control and flooding conditions, peroxidases and glycosyl hydrolases were decreased by 2 days of flooding stress. mRNA expression levels of proteins in the endoplasmic reticulum and N-glycosylation related proteins were downregulated by flooding stress. These results suggest that flooding might negatively affect the process of N-glycosylation of proteins related to stress and protein degradation; however glycoproteins involved in glycolysis are activated.
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影响因子：
2
作者：
Githiri, S. M.;Watanabe, S.;Takahashi, R.
通讯作者：
Takahashi, R.
影响因子：
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