Synaptic Ribbons Require Ribeye for Electron Density, Proper Synaptic Localization, and Recruitment of Calcium Channels.
Synaptic Ribbons Require Ribeye for Electron Density, Proper Synaptic Localization, and Recruitment of Calcium Channels.
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突触带需要 Ribeye 来实现电子密度、正确的突触定位和钙通道的募集。
DOI：
10.1016/j.celrep.2016.05.045
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发表时间：
2016
期刊：
影响因子：
8.8
通讯作者：
Zenisek,David
中科院分区：
文献类型：
--
作者：
Lv,Caixia;Stewart,WilliamJ;Akanyeti,Otar;Frederick,Courtney;Zhu,Jie;Santos-Sacchi,Joseph;Sheets,Lavinia;Liao,JamesC;Zenisek,David
突触带是主要由蛋白Ribeye组成的结构，在某些感觉系统中，它将突触囊泡保持在非尖峰细胞的释放部位附近。在这里，我们引入移码突变的两个斑马鱼基因编码Ribeye，从而删除Ribeye蛋白质从神经肥大毛细胞。尽管Ribeye耗尽，囊泡聚集在缺乏电子密度的带状结构周围，我们称之为“幽灵带”。“鬼带的尺寸较小，但具有类似数量的小泡，并且难以定位于突触和钙通道。这些毛细胞表现出增强的胞吐作用，如通过电容测量的，并且从突触后传入神经元到毛细胞的记录显示尖峰速率没有显著差异。我们的研究结果表明，Ribeye构成了神经肥大毛细胞中大部分的突触带密度，并且对于钙通道和突触带的适当定位是必要的。
Synaptic ribbons are structures made largely of the protein Ribeye that hold synaptic vesicles near release sites in non-spiking cells in some sensory systems. Here, we introduce frameshift mutations in the two zebrafish genes encoding for Ribeye and thus remove Ribeye protein from neuromast hair cells. Despite Ribeye depletion, vesicles collect around ribbon-like structures that lack electron density, which we term "ghost ribbons." Ghost ribbons are smaller in size but possess a similar number of smaller vesicles and are poorly localized to synapses and calcium channels. These hair cells exhibit enhanced exocytosis, as measured by capacitance, and recordings from afferent neurons post-synaptic to hair cells show no significant difference in spike rates. Our results suggest that Ribeye makes up most of the synaptic ribbon density in neuromast hair cells and is necessary for proper localization of calcium channels and synaptic ribbons.
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