Metabolic Fluctuations in the Human Stool Obtained from Blastocystis Carriers and Non-Carriers.
Metabolic Fluctuations in the Human Stool Obtained from Blastocystis Carriers and Non-Carriers.
复制标题
DOI：
10.3390/metabo11120883
复制
发表时间：
2021-12-17
期刊：
影响因子：
4.1
通讯作者：
Tsaousis AD
中科院分区：
文献类型：
--
作者：
Betts EL;Newton JM;Thompson GS;Sarzhanov F;Jinatham V;Kim MJ;Popluechai S;Dogruman-Al F;Won EJ;Gentekaki E;Tsaousis AD
芽囊菌是一种专性厌氧微生物真核生物，经常栖息在胃肠道中。尽管如此普遍，但人们对其遗传多样性、致病性以及与其他微生物组及其宿主的相互作用的程度知之甚少。尽管生物体在形态上是静态的，但它具有不少于 28 个遗传上不同的亚型 (ST)。关于芽囊菌致病性的报道是相互矛盾的。人们越来越多地探索芽囊菌和肠道细菌群落之间的关联。尽管如此，扩展到代谢组的类似研究并不存在。通过对来自韩国 (n = 38)、泰国 (n = 44) 和土耳其 (n = 69) 的 149 份粪便样本建立的 NMR 代谢组学方案，我们提供了含有 (B+) 和不含 (B−) 芽囊藻的人类粪便中存在的核心代谢化合物的快照。样本包括有胃肠道症状和无症状的宿主。在韩国、泰国和土耳其样本集中，总共有 9、62 和 98 种显着代谢物分别与囊胚携带相关，其中一些代谢物在定植组中有所增加。来自所有国家的 B+ 和 B− 样本的代谢特征是不同的，并在偏最小二乘判别分析 (PLS-DA) 中单独分组。典型的炎症相关代谢物与芽囊菌阳性样本呈负相关。这些数据将有助于指导未来有关芽囊菌参与肠道微生物组和宿主生理过程的研究。未来使用代谢组和微生物组数据以及宿主生理学和免疫反应信息的研究将有助于阐明芽囊菌在健康和疾病中的作用。
Blastocystis is an obligate anaerobic microbial eukaryote that frequently inhabits the gastrointestinal tract. Despite this prevalence, very little is known about the extent of its genetic diversity, pathogenicity, and interaction with the rest of the microbiome and its host. Although the organism is morphologically static, it has no less than 28 genetically distinct subtypes (STs). Reports on the pathogenicity of Blastocystis are conflicting. The association between Blastocystis and intestinal bacterial communities is being increasingly explored. Nonetheless, similar investigations extending to the metabolome are non-existent.Using established NMR metabolomics protocols in 149 faecal samples from individuals from South Korea (n = 38), Thailand (n = 44) and Turkey (n = 69), we have provided a snapshot of the core metabolic compounds present in human stools with (B+) and without (B−) Blastocystis. Samples included hosts with gastrointestinal symptoms and asymptomatics. A total of nine, 62 and 98 significant metabolites were associated with Blastocystis carriage in the South Korean, Thai and Turkish sample sets respectively, with a number of metabolites increased in colonised groups. The metabolic profiles of B+ and B− samples from all countries were distinct and grouped separately in the partial least squares-discriminant analysis (PLS-DA). Typical inflammation-related metabolites negatively associated with Blastocystis positive samples. This data will assist in directing future studies underlying the involvement of Blastocystis in physiological processes of both the gut microbiome and the host. Future studies using metabolome and microbiome data along with host physiology and immune responses information will contribute significantly towards elucidating the role of Blastocystis in health and disease.
登录
查看更多内容
影响因子：
3.7
作者：
Jeelani G;Sato D;Husain A;Escueta-de Cadiz A;Sugimoto M;Soga T;Suematsu M;Nozaki T
通讯作者：
Nozaki T
影响因子：
5.2
作者：
Billy V;Lhotská Z;Jirků M;Kadlecová O;Frgelecová L;Parfrey LW;Pomajbíková KJ
通讯作者：
Pomajbíková KJ
影响因子：
4.2
作者：
Betts EL;Hoque S;Torbe L;Bailey JR;Ryan H;Toller K;Breakell V;Carpenter AI;Diana A;Matechou E;Gentekaki E;Tsaousis AD
通讯作者：
Tsaousis AD
DOI：
10.1097/mpg.0000000000001812
发表时间：
2018-05-01
影响因子：
2.9
作者：
Bosch, Sofie;Struys, Eduard A.;de Meij, Tim G. J.
通讯作者：
de Meij, Tim G. J.
影响因子：
9.8
作者：
Gentekaki E;Curtis BA;Stairs CW;Klimeš V;Eliáš M;Salas-Leiva DE;Herman EK;Eme L;Arias MC;Henrissat B;Hilliou F;Klute MJ;Suga H;Malik SB;Pightling AW;Kolisko M;Rachubinski RA;Schlacht A;Soanes DM;Tsaousis AD;Archibald JM;Ball SG;Dacks JB;Clark CG;van der Giezen M;Roger AJ
通讯作者：
Roger AJ