Parasites, Drugs and Captivity: Blastocystis-Microbiome Associations in Captive Water Voles.
Parasites, Drugs and Captivity: Blastocystis-Microbiome Associations in Captive Water Voles.
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DOI：
10.3390/biology10060457
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发表时间：
2021-05-22
期刊：
影响因子：
4.2
通讯作者：
Tsaousis AD
中科院分区：
文献类型：
--
作者：
Betts EL;Hoque S;Torbe L;Bailey JR;Ryan H;Toller K;Breakell V;Carpenter AI;Diana A;Matechou E;Gentekaki E;Tsaousis AD
在过去的十年里，人们对微生物群及其在健康和疾病中的作用的研究有了很大的增加。这项工作的大部分主要集中在微生物组的细菌成分上。然而，有证据表明，定植胃肠道的微生物真核生物可能在微生物群的形成和结构中发挥作用，因此可能影响疾病结局和宿主健康。本研究的目的是调查可疑病原体囊虫，并扩大对非灵长类宿主的研究数量，以解决其与肠道细菌群落的关系。在此，我们检测了囊胚阳性和阴性水田鼠的肠道微生物群。结果显示囊虫、细菌丰富度和群落组成没有相关性。尽管如此，在囊胚菌阳性样品中，一些分类群的丰度受到影响。囊胚菌携带者和非携带者之间的群落丰度缺乏显著变化，表明这种微生物可能不会对这些动物肠道中的细菌群落产生深远影响。(1)背景：囊虫是一种寄生于包括人类在内的多种动物胃肠道的真核微生物。几项研究表明，该有机体与被认为对肠道和整体健康有益的特定微生物概况和细菌分类群有关。尽管如此，这些研究几乎完全集中在人类身上，而在其他动物身上没有类似的信息。(2)方法：采用常规PCR、克隆和测序相结合的方法，对某野生动物园2次采集的16只圈养水田鼠进行了囊虫、贾第鞭毛虫和隐孢子虫的检测。我们还描述了他们的肠道细菌群落。(3)结果：总体而言，携带囊胚和未携带囊胚的水鼠α和β多样性差异不显著。在囊胚孢子虫阳性的水田鼠中，密螺旋体和动丝虫的数量明显减少，仅在单个分类群上存在差异。根据抗原虫治疗和其他原生生物的存在进行分组，细菌群落组成也没有任何差异。(4)结论：与人类的研究不同，囊虫似乎与水田鼠的特定肠道微生物特征无关。
The last decade has seen a large increase into research on the microbiome and its roles in health and disease. The majority of this work has focused primarily on the bacterial component of the microbiome. However, there is evidence to suggest that microbial eukaryotes colonising the gastrointestinal tract may have roles in the shaping and structuring of the microbiota and are thus likely to influence disease outcomes and host health. The aim of this study was to investigate the questionable pathogen Blastocystis and expand the number of studies on non-primate hosts, which address its associations with bacterial communities in the gut. Herein we examined the bacterial gut microbiota of Blastocystis positive and negative water voles. Results demonstrate no association of Blastocystis, bacterial richness and community composition. Nonetheless, the abundance of some taxa was affected in Blastocystis positive samples. The lack of significant shifts in community abundance between Blastocystis carriers and non-carriers indicates that this microbe may not be having a profound impact on bacterial communities in the gut of these animals. (1) Background: Blastocystis is a microbial eukaryote inhabiting the gastrointestinal tract of a broad range of animals including humans. Several studies have shown that the organism is associated with specific microbial profiles and bacterial taxa that have been deemed beneficial to intestinal and overall health. Nonetheless, these studies are focused almost exclusively on humans, while there is no similar information on other animals. (2) Methods: Using a combination of conventional PCR, cloning and sequencing, we investigated presence of Blastocystis along with Giardia and Cryptosporidium in 16 captive water voles sampled twice from a wildlife park. We also characterised their bacterial gut communities. (3) Results: Overall, alpha and beta diversities between water voles with and without Blastocystis did not differ significantly. Differences were noted only on individual taxa with Treponema and Kineothrix being significantly reduced in Blastocystis positive water voles. Grouping according to antiprotozoal treatment and presence of other protists did not reveal any differences in the bacterial community composition either. (4) Conclusion: Unlike human investigations, Blastocystis does not seem to be associated with specific gut microbial profiles in water voles.
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影响因子：
12.2
作者：
Čoklo M;Maslov DR;Kraljević Pavelić S
通讯作者：
Kraljević Pavelić S
影响因子：
64.8
作者：
Arumugam, Manimozhiyan;Raes, Jeroen;Pelletier, Eric;Le Paslier, Denis;Yamada, Takuji;Mende, Daniel R.;Fernandes, Gabriel R.;Tap, Julien;Bruls, Thomas;Batto, Jean-Michel;Bertalan, Marcelo;Borruel, Natalia;Casellas, Francesc;Fernandez, Leyden;Gautier, Laurent;Hansen, Torben;Hattori, Masahira;Hayashi, Tetsuya;Kleerebezem, Michiel;Kurokawa, Ken;Leclerc, Marion;Levenez, Florence;Manichanh, Chaysavanh;Nielsen, H. Bjorn;Nielsen, Trine;Pons, Nicolas;Poulain, Julie;Qin, Junjie;Sicheritz-Ponten, Thomas;Tims, Sebastian;Torrents, David;Ugarte, Edgardo;Zoetendal, Erwin G.;Wang, Jun;Guarner, Francisco;Pedersen, Oluf;de Vos, Willem M.;Brunak, Soren;Dore, Joel;Weissenbach, Jean;Ehrlich, S. Dusko;Bork, Peer
通讯作者：
Bork, Peer
影响因子：
1.5
作者：
Clark, CG
通讯作者：
Clark, CG
微生物群-肠-脑相互作用调节雄性布氏田鼠（Lasiopodomysbrandtii）对寒冷的宿主代谢适应
影响因子：
11
作者：
Bo, Ting-Bei;Zhang, Xue-Ying;Wang, De-Hua
通讯作者：
Wang, De-Hua
影响因子：
15.5
作者：
Angly FE;Dennis PG;Skarshewski A;Vanwonterghem I;Hugenholtz P;Tyson GW
通讯作者：
Tyson GW