Metabolic profiling of the protozoan parasite Entamoeba invadens revealed activation of unpredicted pathway during encystation.
Metabolic profiling of the protozoan parasite Entamoeba invadens revealed activation of unpredicted pathway during encystation.
复制标题
DOI：
10.1371/journal.pone.0037740
复制
发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Nozaki T
中科院分区：
文献类型：
--
作者：
Jeelani G;Sato D;Husain A;Escueta-de Cadiz A;Sugimoto M;Soga T;Suematsu M;Nozaki T
包囊形成是细胞从活动的、增殖的、不稳定的滋养体形式分化为休眠的、抗性包囊形式，是在寄生和自由生活的原生动物如内阿米巴、贾第虫、阿米巴和Balamuthia中发现的关键过程。由于成囊是生命周期中应对不利外部环境变化的重要过程，并且通常与疾病的传播不可或缺，因此对该过程的生物化学理解可能提供有用的措施来对抗由这组原生动物引起的感染。在这项研究中，我们研究了在包囊内发生的代谢和转录组的变化，在入侵内阿米巴，爬行动物的兄弟哺乳动物感染E。histolytica，使用基于毛细管电泳-串联质谱的代谢物分析和基于DNA微阵列的表达分析。随着包囊形成的进展，参与糖酵解的大多数代谢物和核苷酸的水平急剧下降，表明能量产生停止。此外，糖酵解通量被重定向到几丁质壁的生物合成。我们发现显着的时间增加生物胺，如异戊胺，异丁胺，尸胺，在早期的包囊，当滋养体形成大的多细胞聚集体（precysts）。我们还发现在成囊过程中γ-氨基丁酸（GABA）有明显的诱导作用。这项研究首次揭示了包囊形成过程中转录和代谢调控网络的动态，有助于更好地了解致病性真核生物的过程，并进一步制定控制感染的措施。
Encystation, which is cellular differentiation from the motile, proliferative, labile trophozoite form to the dormant, resistant cyst form, is a crucial process found in parasitic and free-living protozoa such as Entamoeba, Giardia, Acanthamoeba, and Balamuthia. Since encystation is an essential process to deal with the adverse external environmental changes during the life cycle, and often integral to the transmission of the diseases, biochemical understanding of the process potentially provides useful measures against the infections caused by this group of protozoa. In this study, we investigated metabolic and transcriptomic changes that occur during encystation in Entamoeba invadens, the reptilian sibling of mammal-infecting E. histolytica, using capillary electrophoresis-tandem mass spectrometry-based metabolite profiling and DNA microarray-based expression profiling. As the encystation progressed, the levels of majority of metabolites involved in glycolysis and nucleotides drastically decreased, indicating energy generation is ceased. Furthermore, the flux of glycolysis was redirected toward chitin wall biosynthesis. We found remarkable temporal increases in biogenic amines such as isoamylamine, isobutylamine, and cadaverine, during the early period of encystation, when the trophozoites form large multicellular aggregates (precyst). We also found remarkable induction of γ-aminobutyric acid (GABA) during encystation. This study has unveiled for the first time the dynamics of the transcriptional and metabolic regulatory networks during encystation, and should help in better understanding of the process in pathogenic eukaryotes, and further development of measures controlling infections they cause.
登录
查看更多内容
影响因子：
2.9
作者：
BADET, B;VERMOOTE, P;LEGOFFIC, F
通讯作者：
LEGOFFIC, F
影响因子：
3.5
作者：
CHAPMAN, A;LINSTEAD, DJ;WILLIAMS, J
通讯作者：
WILLIAMS, J
影响因子：
64.8
作者：
Loftus, B;Anderson, I;Hall, N
通讯作者：
Hall, N
影响因子：
7.4
作者：
Bölling, C;Fiehn, O
通讯作者：
Fiehn, O
影响因子：
4.8
作者：
Husain, Afzal;Sato, Dan;Nozaki, Tomoyoshi
通讯作者：
Nozaki, Tomoyoshi