Preliminary Study on Hepatocyte-Targeted Phosphorus-31 MRS Using ATP-Loaded Galactosylated Chitosan Oligosaccharide Nanoparticles.
Preliminary Study on Hepatocyte-Targeted Phosphorus-31 MRS Using ATP-Loaded Galactosylated Chitosan Oligosaccharide Nanoparticles.
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DOI：
10.1155/2013/512483
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发表时间：
2013
影响因子：
2
通讯作者：
Du YZ
中科院分区：
文献类型：
--
作者：
Yu RS;Zhu XL;Sun JZ;Shi D;Chen Y;Wang ZK;Tang KZ;Du YZ
背景由于正常肝组织和病理组织中磷酸盐的变化不明显，肝细胞靶向31 P磁共振波谱（31 P MRS）的研究文献较少，因此31 P MRS的临床应用仍存在一定的困难。制备了三磷酸腺苷（ATP）修饰的半乳糖壳聚糖（Gal-CSO）纳米粒，并分别用HepG-2肿瘤细胞和A549肿瘤细胞研究了其特异性细胞摄取。将两种细胞分别与纳米颗粒悬浮液孵育。然后制备细胞样品，评价31 P MRS检测到的ATP峰的增强效率。结果HepG-2细胞对Gal-CSO/ATP纳米粒的摄取率高于A549细胞。此外，31 P MRS检测到Gal-CSO/ATP纳米粒在体外HepG-2细胞中的ATP放大峰高于A549细胞。Gal-CSO/ATP纳米粒在体外对肝细胞具有明显的靶向性，对HepG-2细胞中ATP峰具有明显的增强作用。31 P MRS可用于肝脏分子影像学研究。
Background. The clinical applications of hepatic phosphorus-31 magnetic resonance spectroscopy (31P MRS) remain to be difficult because the changes of phosphates between normal hepatic tissues and pathological tissues are not so obvious, and furthermore, up to now there is few literature on hepatocyte-targeted 31P MRS. Materials and Methods. The ATP-loaded Gal-CSO (Gal-CSO/ATP) nanoparticles were prepared and the special cellular uptake of them as evaluated by using HepG-2 tumor cells and A549 tumor cells, respectively. Two kinds of cells were incubated with the nanoparticles suspension, respectively. Then were prepared the cell samples and the enhancement efficiency of ATP peaks detected by 31P MRS was evaluated. Results. The cellular uptake rate of Gal-CSO/ATP nanoparticles in HepG-2 cells was higher than that in A549 cells. Furthermore, the enlarged ATP peaks of Gal-CSO/ATP nanoparticles in HepG-2 cells were higher than those in A549 cells in vitro detected by 31P MRS. Conclusions. Gal-CSO/ATP nanoparticles have significant targeting efficiency in hepatic cells in vitro and enhancement efficiency of ATP peaks in HepG-2 cells. Furthermore, 31P MRS could be applied in the research of hepatic molecular imaging.
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影响因子：
2.9
作者：
Morse, David L.;Carroll, Danielle;Day, Sam;Gray, Heather;Sadarangani, Pooja;Murthi, Shiva;Job, Constantin;Baggett, Brenda;Raghunand, Natarajan;Gillies, Robert J.
通讯作者：
Gillies, Robert J.
影响因子：
13.5
作者：
Corbin, IR;Buist, R;Minuk, GY
通讯作者：
Minuk, GY
影响因子：
1.3
作者：
Kugel, H;Wittsack, HJ;Lackner, K
通讯作者：
Lackner, K
负载多西紫杉醇的固体脂质纳米粒靶向肝细胞癌的性能。
影响因子：
14
作者：
Xu, Zhenghong;Chen, Lingli;Li, Yaping
通讯作者：
Li, Yaping
影响因子：
4.8
作者：
Yik, JHN;Saxena, A;Weigel, PH
通讯作者：
Weigel, PH