Dissemination and transmission of the E1-226V variant of chikungunya virus in Aedes albopictus are controlled at the midgut barrier level.
Dissemination and transmission of the E1-226V variant of chikungunya virus in Aedes albopictus are controlled at the midgut barrier level.
复制标题
DOI：
10.1371/journal.pone.0057548
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Failloux AB
中科院分区：
文献类型：
--
作者：
Arias-Goeta C;Mousson L;Rougeon F;Failloux AB
虫媒病毒的出现可能是因为它们有能力利用新的环境，例如新的宿主。这种能力是由病毒基因组复制过程中发生的高突变率促进的。印度洋地区最后一次出现基孔肯雅热证实了这一说法，因为在主要蚊媒埃及伊蚊不存在的地区，E1糖蛋白(E1-A226V)第226位的单一病毒突变与白纹伊蚊的传播增强有关。我们使用体内直接竞争分析来剖析Ae选择E1-226V的机制。白纹伊蚊。将原来的变异体E1-226A和新出现的变异体E1-226V以相同的滴度提供给两种蚊子的同一血粉中，我们发现两种蚊子中这两种变异体的比例有很大差异。在摄入传染性血粉后，在Ae中优先选择了E1-226V变异体。白纹伊蚊，而E1-226A变异体有时在Ae.埃及伊蚊。有趣的是，当这两个变种通过胸腔接种进入蚊子后，E1-226V不再适合在Ae中传播和传播。白纹伊蚊，表明中肠屏障在E1-226V的选择中起着关键作用。这项研究揭示了中肠屏障在选择新的虫媒病毒新变种中的作用。我们还带来了新的见解，以了解如何在包括E1-226A在内的其他病毒变种中选择先前存在的变种E1-226V。事实上，在自然病毒种群中以低水平存在的E1-226V变异株在被选入Ae后可能会迅速出现。白纹伊蚊在中肠屏障水平。
Emergence of arboviruses could result from their ability to exploit new environments, for example a new host. This ability is facilitated by the high mutation rate occurring during viral genome replication. The last emergence of chikungunya in the Indian Ocean region corroborates this statement since a single viral mutation at the position 226 on the E1 glycoprotein (E1-A226V) was associated with enhanced transmission by the mosquito Aedes albopictus in regions where the major mosquito vector, Aedes aegypti, is absent. We used direct competition assays in vivo to dissect out the mechanisms underlying the selection of E1-226V by Ae. albopictus. When the original variant E1-226A and the newly emerged E1-226V were provided in the same blood-meal at equal titers to both species of mosquitoes, we found that the proportion of both variants was drastically different in the two mosquito species. Following ingestion of the infectious blood-meal, the E1-226V variant was preferentially selected in Ae. albopictus, whereas the E1-226A variant was sometimes favored in Ae. aegypti. Interestingly, when the two variants were introduced into the mosquitoes by intrathoracic inoculations, E1-226V was no longer favored for dissemination and transmission in Ae. albopictus, showing that the midgut barrier plays a key role in E1-226V selection. This study sheds light on the role of the midgut barrier in the selection of novel arbovirus emerging variants. We also bring new insight into how the pre-existing variant E1-226V was selected among other viral variants including E1-226A. Indeed the E1-226V variant present at low levels in natural viral populations could rapidly emerge after being selected in Ae. albopictus at the midgut barrier level.
登录
查看更多内容
影响因子：
3.7
作者：
Tsetsarkin KA;McGee CE;Volk SM;Vanlandingham DL;Weaver SC;Higgs S
通讯作者：
Higgs S
影响因子：
3.7
作者：
Vazeille, Marie;Moutailler, Sara;Coudrier, Daniel;Rousseaux, Claudine;Khun, Huot;Huerre, Michel;Thiria, Julien;Dehecq, Jean-Sebastien;Fontenille, Didier;Schuffenecker, Isabelle;Despres, Philippe;Failloux, Anna-Bella
通讯作者：
Failloux, Anna-Bella
DOI：
10.4269/ajtmh.1974.23.1153
发表时间：
1974-01-01
影响因子：
3.3
作者：
ROSEN, L;GUBLER, D
通讯作者：
GUBLER, D
影响因子：
2.1
作者：
Delatte, H.;Dehecq, J. S.;Fontenille, D.
通讯作者：
Fontenille, D.
影响因子：
--
作者：
Martin E;Moutailler S;Madec Y;Failloux AB
通讯作者：
Failloux AB