Overexpression of ferredoxin, PETF, enhances tolerance to heat stress in Chlamydomonas reinhardtii.
Overexpression of ferredoxin, PETF, enhances tolerance to heat stress in Chlamydomonas reinhardtii.
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DOI：
10.3390/ijms141020913
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发表时间：
2013-10-17
影响因子：
5.6
通讯作者：
Huang LF
中科院分区：
文献类型：
--
作者：
Lin YH;Pan KY;Hung CH;Huang HE;Chen CL;Feng TY;Huang LF
植物在恶劣环境中产生的活性氧(ROS)会对细胞器造成损害，引发细胞死亡。清除过量的ROS可以通过抗坏血酸清除剂途径来实现，以防止植物细胞死亡。这种清道夫的量可以由铁氧还蛋白(FDX)调节。绿色体FDX是一种电子转移蛋白，在分配光合作用还原能力方面发挥作用。在这项研究中，我们证明了在正常生长条件下，内源光合作用FDX基因PETF在莱茵衣藻中的过表达可以提高还原型抗坏血酸水平，降低H_2O_2水平。此外，高表达PETF转基因衣藻产生低水平的过氧化氢，并表现出保护作用，这是通过减少叶绿素降解和提高细胞在热胁迫条件下的存活率而观察到的。这项研究的结果表明，过表达PETF可以提高叶绿体清除ROS的效率，从而赋予植物耐热性。PETF在ROS水平下调中的作用为潜在地提高作物对高温胁迫的耐受性提供了一种方法。
Reactive oxygen species (ROS) produced by plants in adverse environments can cause damage to organelles and trigger cell death. Removal of excess ROS can be achieved through the ascorbate scavenger pathway to prevent plant cell death. The amount of this scavenger can be regulated by ferredoxin (FDX). Chloroplastic FDXs are electron transfer proteins that perform in distributing photosynthetic reducing power. In this study, we demonstrate that overexpression of the endogenous photosynthetic FDX gene, PETF, in Chlamydomonas reinhardtii could raise the level of reduced ascorbate and diminish H2O2 levels under normal growth conditions. Furthermore, the overexpressing PETF transgenic Chlamydomonas lines produced low levels of H2O2 and exhibited protective effects that were observed through decreased chlorophyll degradation and increased cell survival under heat-stress conditions. The findings of this study suggest that overexpression of PETF can increase the efficiency of ROS scavenging in chloroplasts to confer heat tolerance. The roles of PETF in the downregulation of the ROS level offer a method for potentially improving the tolerance of crops against heat stress.
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影响因子：
3.9
作者：
Kavitha, Kumaresan;George, Suja;Parida, Ajay
通讯作者：
Parida, Ajay
DOI：
10.1007/978-1-59745-365-3_10
发表时间：
2007-01-01
期刊：
Methods in molecular biology (Clifton, N.J.)
影响因子：
--
作者：
Funes, Soledad;Franzen, Lars-Gunnar;Gonzalez-Halphen, Diego
通讯作者：
Gonzalez-Halphen, Diego
影响因子：
3.7
作者：
Allakhverdiev, Suleyman I.;Murata, Norio
通讯作者：
Murata, Norio
影响因子：
11.6
作者：
Davletova, S;Rizhsky, L;Mittler, R
通讯作者：
Mittler, R
影响因子：
6.4
作者：
Galvez-Valdivieso, Gregorio;Mullineaux, Philip M.
通讯作者：
Mullineaux, Philip M.