Plant-Produced Antigen Displaying Virus-Like Particles Evokes Potent Antibody Responses against West Nile Virus in Mice.
Plant-Produced Antigen Displaying Virus-Like Particles Evokes Potent Antibody Responses against West Nile Virus in Mice.
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DOI：
10.3390/vaccines9010060
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发表时间：
2021-01-17
期刊：
影响因子：
7.8
通讯作者：
Chen Q
中科院分区：
文献类型：
--
作者：
He J;Lai H;Esqueda A;Chen Q
在这项研究中，我们开发了一种基于B型肝炎核心抗原（HBcAg）的病毒样颗粒（VLP），它展示了西尼罗河病毒（WNV）包膜蛋白结构域III（wDIII）作为WNV的候选疫苗。HBcAg-wDIII融合蛋白在本氏烟草植物中快速产生，并在基因渗入后6天内达到约1.2mg融合蛋白/克叶鲜重的高表达水平。电子显微镜和梯度离心分析表明，wDIII的引入不干扰VLP的形成和HBcAg-wDIII成功组装成VLP。HBcAg-wDIII VLP可以容易地从本氏烟草叶中大量纯化至>95%的均一性。进一步的分析显示，wDIII被正确地展示，并显示出与识别wDIII构象表位的抗wDIII单克隆抗体的特异性结合。值得注意的是，HBcAg-wDIII VLP被证明具有高度免疫原性，并在小鼠中引发有效的体液应答，其抗原特异性IgG滴度相当于先前研究中保护性wDIII抗原的滴度。因此，我们的基于wDIII的VLP疫苗为开发针对WNV的有效、安全和低成本疫苗提供了有吸引力的选择。
In this study, we developed a hepatitis B core antigen (HBcAg)-based virus-like particle (VLP) that displays the West Nile virus (WNV) Envelope protein domain III (wDIII) as a vaccine candidate for WNV. The HBcAg-wDIII fusion protein was quickly produced in Nicotiana benthamiana plants and reached a high expression level of approximately 1.2 mg of fusion protein per gram of leaf fresh weight within six days post gene infiltration. Electron microscopy and gradient centrifugation analysis indicated that the introduction of wDIII did not interfere with VLP formation and HBcAg-wDIII successfully assembled into VLPs. HBcAg-wDIII VLPs can be easily purified in large quantities from Nicotiana benthamiana leaves to >95% homogeneity. Further analysis revealed that the wDIII was displayed properly and demonstrated specific binding to an anti-wDIII monoclonal antibody that recognizes a conformational epitope of wDIII. Notably, HBcAg-wDIII VLPs were shown to be highly immunogenic and elicited potent humoral responses in mice with antigen-specific IgG titers equivalent to that of protective wDIII antigens in previous studies. Thus, our wDIII-based VLP vaccine offers an attractive option for developing effective, safe, and low-cost vaccines against WNV.
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影响因子：
5.5
作者：
Heinz, Franz X.;Holzmann, Heidemarie;Kundi, Michael
通讯作者：
Kundi, Michael
DOI：
10.1007/978-1-4939-3289-4_4
发表时间：
2016-01-01
期刊：
RECOMBINANT PROTEINS FROM PLANTS: METHODS AND PROTOCOLS, 2ND EDITION
影响因子：
--
作者：
Chen, Qiang;Dent, Matthew;Lai, Huafang
通讯作者：
Lai, Huafang
植物生产的抗登革病毒单克隆抗体表现出较低的抗体依赖性感染活性增强效应
影响因子：
3.8
作者：
Dent, Matthew;Hurtado, Jonathan;Chen, Qiang
通讯作者：
Chen, Qiang
影响因子：
13.8
作者：
Hurtado, Jonathan;Acharya, Dhiraj;Chen, Qiang
通讯作者：
Chen, Qiang
影响因子：
5.4
作者：
Crill, WD;Chang, GJJ
通讯作者：
Chang, GJJ